Tested Applications
| Positive WB detected in | mouse pancreas tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17245-1-AP targets SEC16B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11173 Product name: Recombinant human SEC16B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-352 aa of BC009106 Sequence: MELWAPQRLPQTRGKATAPSKDPDRGFRRDGHHRPVPHSWHNGERFHQWQDNRGSPQPQQEPRADHQQQPHYASRPGDWHQPVSGVDYYEGGYRNQLYSRPGYENSYQSYQSPTMREEYAYGSYYYHGHPQWLQEERVPRQRSPYIWHEDYREQKYLDEHHYENQHSPFGTNSETHFQSNSRNPCKDSPASNSGQEWPGELFPGSLLAEAQKNKPSLASESNLLQQRESGLSSSSYELSQYIRDAPERDDPPASAAWSPVQAEDVSSAGPKAPMKFYIPHVPVSFGPGGQLVRVGPSSPTDGQAALVELHSMEVILNDSEEQEEMRSFSGPLIREDVHKVDIMTFCQQKAAQ Predict reactive species |
| Full Name | SEC16 homolog B (S. cerevisiae) |
| Calculated Molecular Weight | 71 kDa, 117 kDa |
| Observed Molecular Weight | 60-70 kDa |
| GenBank Accession Number | BC009106 |
| Gene Symbol | SEC16B |
| Gene ID (NCBI) | 89866 |
| RRID | AB_3085519 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96JE7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SEC16B also known as LZTR2, RGPR-p117, is a mammalian homolog of S. cerevisiae Sec16 that is required for organization of transitional endoplasmic reticulum (ER) sites and protein export (PMID: 17192411). SEC16B regulates the transport of peroxisomal biogenesis factors PEX3 and PEX16 from the ER to peroxisomes (PMID:21768384). SEC16B may be involved in human obesity to possess an association between single nucleotide polymorphisms and different measures of obesity (PMID: 28413634).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SEC16B antibody 17245-1-AP | Download protocol |
| WB protocol for SEC16B antibody 17245-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SEC16B Polyclonal antibody (Cat# 17245-1-AP) has 1 citation to date. It was cited in EMBO Journal (Impact Factor 8.4) in a study investigating how the tissue-selective COPII modulator SEC16B aggravates cardiovascular disease by promoting lipid export. The research field encompasses cardiovascular biology and lipid metabolism, with the antibody applied in Western blot using mouse knockout models.
| Species | Application | Title |
|---|---|---|
EMBO J Tissue-selective COPII modulator SEC16B aggravates cardiovascular disease by promoting lipid export.
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