SEC23B Polyclonal antibody

SEC23B Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 30510-1-AP

Synonyms

SEC23 related protein B, SEC23B

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Validation Data Gallery

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  WB analysis using 30510-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30510-1-AP (SEC23B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 30510-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using SEC23B antibody (30510-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, MCF-7 cells, Raji cells
• Positive IF detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30510-1-AP targets SEC23B in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEC23B fusion protein Ag33302
• Full Name: Sec23 homolog B (S. cerevisiae)
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 80-86 kDa
• GenBank Accession Number: BC005404
• Gene Symbol: SEC23B
• Gene ID (NCBI): 10483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.