SEC24A Polyclonal antibody

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SEC24A Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15958-1-AP Copied!

Publications (18)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF, IP, ELISA

Synonyms

SEC24 related protein A, SEC24A

• 

  WB analysis of HeLa using 15958-1-AP

  WB result of SEC24A antibody (15958-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEC24A transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 15958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat thymus using 15958-1-AP

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 15958-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse skeletal muscle using 15958-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse thymus using 15958-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lymphoma using 15958-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15958-1-AP (SEC24A antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lymphoma using 15958-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15958-1-AP (SEC24A antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: COS-7 cells, HeLa cells, human liver tissue, mouse skeletal muscle tissue, mouse thymus tissue, rat thymus tissue, HEK-293 cells, HepG2 cells, NIH/3T3 cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15958-1-AP targets SEC24A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8723

  Product name: Recombinant human SEC24A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 264-613 aa of BC019341

  Sequence: DEIEGGGLLATPQLTNKNPKMSRSVGYSYPSLPPGYQNITPPGATGVPPSSLNYPSGPQAFTQTPLGANHLTTSMSGLSLQPEGLRVVNLLQERNMLPSTPLKPPVPNLHEDIQKLNCNPELFRCTLTSIPQMQALLNKAKLPLGLLLHPFKDLVQLPVVTSSTIVRCRSCRTYINPFVSFLDQRRWKCNLCYRVNDVPEEFLYNPLTRVYGEPHRRPEVQNATIEFMAPSEYMLRPPQPPVYLFVFDVSHNAVETGYLNSVCQSLLDNLDLLPGNTRTKIGFITFDSTIHFYGLQESLSQPQMLIVSDIEDVFIPMPENLLVNLNESKESVIGVSSEETLITCLEIAMR

• Full Name: SEC24 family, member A (S. cerevisiae)
• Calculated Molecular Weight: 613 aa, 66 kDa
• Observed Molecular Weight: 66 kDa, 120 kDa
• GenBank Accession Number: BC019341
• Gene Symbol: SEC24A
• Gene ID (NCBI): 10802
• RRID: AB_2186118
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95486
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445). Two isoforms of 120 kDa and 66 kDa produced by alternative splicing have been described.

Publications

What published studies show

The SEC24A Polyclonal antibody (Cat# 15958-1-AP) has 18 citations spanning journals including Nature, Cell, Cell Metabolism, JCI, Developmental Cell, Advanced Science, and others. Research fields cover COPII vesicle trafficking, hepatic lipid metabolism, cholesterol homeostasis, STING signaling, autophagy, antitumor immunity, neuronal differentiation, ER stress, viral infection, and gastric cancer. Applications cited include WB, IF, IP, and IHC across human, mouse, rat, and monkey species.

Automatically generated from the 18 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nature; The CREB coactivator CRTC2 controls hepatic lipid metabolism by regulating SREBP1.; Authors - Jinbo Han
mouse,human	WB	Cell; STING signaling modulation by COPII cargo recognition.; Authors - Heng Lyu ; KO Validated
human	IF	Cell Metab; The hypercholesterolemia-risk gene SORT1 facilitates PCSK9 secretion.; Authors - Camilla Gustafsen
human,mouse	WB,IF	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
mouse	WB,IF	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
mouse	WB	J Clin Invest; The COPII cargo adapter SEC24C is essential for neuronal homeostasis.; Authors - Bo Wang