SEC24A Polyclonal antibody

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  WB analysis of HeLa using 15958-1-AP

  WB result of SEC24A antibody (15958-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEC24A transfected HeLa cells.

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  WB analysis using 15958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat thymus using 15958-1-AP

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 15958-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15958-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 15958-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15958-1-AP (SEC24A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 15958-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15958-1-AP (SEC24A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 15958-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15958-1-AP (SEC24A antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

SEC24A Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15958-1-AP

Publications(15)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF, IP, ELISA

Synonyms

SEC24 related protein A, SEC24A

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Tested Applications

• Positive WB detected in: COS-7 cells, HeLa cells, human liver tissue, mouse skeletal muscle tissue, mouse thymus tissue, rat thymus tissue, HEK-293 cells, HepG2 cells, NIH/3T3 cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Product Information

15958-1-AP targets SEC24A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8723

  Product name: Recombinant human SEC24A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 264-613 aa of BC019341

  Sequence: DEIEGGGLLATPQLTNKNPKMSRSVGYSYPSLPPGYQNITPPGATGVPPSSLNYPSGPQAFTQTPLGANHLTTSMSGLSLQPEGLRVVNLLQERNMLPSTPLKPPVPNLHEDIQKLNCNPELFRCTLTSIPQMQALLNKAKLPLGLLLHPFKDLVQLPVVTSSTIVRCRSCRTYINPFVSFLDQRRWKCNLCYRVNDVPEEFLYNPLTRVYGEPHRRPEVQNATIEFMAPSEYMLRPPQPPVYLFVFDVSHNAVETGYLNSVCQSLLDNLDLLPGNTRTKIGFITFDSTIHFYGLQESLSQPQMLIVSDIEDVFIPMPENLLVNLNESKESVIGVSSEETLITCLEIAMR

• Full Name: SEC24 family, member A (S. cerevisiae)
• Calculated Molecular Weight: 613 aa, 66 kDa
• Observed Molecular Weight: 66 kDa, 120 kDa
• GenBank Accession Number: BC019341
• Gene Symbol: SEC24A
• Gene ID (NCBI): 10802
• RRID: AB_2186118
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95486
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445). Two isoforms of 120 kDa and 66 kDa produced by alternative splicing have been described.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nature; The CREB coactivator CRTC2 controls hepatic lipid metabolism by regulating SREBP1.; Authors - Jinbo Han
human	IF	Cell Metab; The hypercholesterolemia-risk gene SORT1 facilitates PCSK9 secretion.; Authors - Camilla Gustafsen
mouse	WB	J Clin Invest; The COPII cargo adapter SEC24C is essential for neuronal homeostasis.; Authors - Bo Wang
mouse	WB,IF	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
human,mouse	WB,IF	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
human	WB,IF,IP	J Immunother Cancer; THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy.; Authors - Chushu Li ; KD Validated