SEC6 Polyclonal antibody, PBS Only

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• SEC6 Polyclonal antibody, PBS Only (14703-1-PBS)

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  WB analysis of human brain using 14703-1-AP (same clone as 14703-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:86 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

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  WB analysis using 14703-1-AP (same clone as 14703-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14703-1-AP (same clone as 14703-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 14703-1-AP (same clone as 14703-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14703-1-AP (same clone as 14703-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14703-1-AP (same clone as 14703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14703-1-AP (same clone as 14703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 14703-1-AP (same clone as 14703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14703-1-AP (SEC6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14703-1-AP (same clone as 14703-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 14703-1-AP (SEC6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SEC6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 14703-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EXOC3, Exocyst complex component 3, Exocyst complex component Sec6, SEC6L1

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Product Information

14703-1-PBS targets SEC6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6383

  Product name: Recombinant human SEC6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 381-745 aa of BC064569

  Sequence: DTYMSTLTSNIIAWLRKALETDKKDWVKETEPEADQDGYYQTTLPAIVFQMFEQNLQVAAQISEDLKTKVLVLCLQQMNSFLSRYKDEAQLYKEEHLRNRQHPHCYVQYMIAIINNCQTFKESIVSLKRKYLKNEVEEGVSPSQPSMDGILDAIAKEGCSGLLEEVFLDLEQHLNELMTKKWLLGSNAVDIICVTVEDYFNDFAKIKKPYKKRMTAEAHRRVVVEYLRAVMQKRISFRSPEERKEGAEKMVREAEQLRFLFRKLASGFGEDVDGYCDTIVAVAEVIKLTDPSLLYLEVSTLVSKYPDIRDDHIGALLAVRGDASRDMKQTIMETLEQGPAQASPSYVPLFKDIVVPSLNVAKLLK

• Full Name: exocyst complex component 3
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC064569
• Gene Symbol: SEC6
• Gene ID (NCBI): 11336
• RRID: AB_2101542
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60645
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Exoc3 (Sec6) is a component of the exocyst (Sec6/8) complex involved in vesicle trafficking at neural synapses and vesicle targeting in polarized epithelial cells. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. This complex is composed of eight proteins (Sec3, Sec5, Sec6, Sec8, Sec10, and Sec15, Exo70 and Exo84), with molecular weights ranging from 70 to 144 kDa.