SEC62 Polyclonal antibody

SEC62 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 28693-1-AP

Synonyms

Dtrp1, FLJ32803, hTP 1, HTP1, SEC62, SEC62 homolog (S. cerevisiae), TLOC1, TP 1, Translocation protein 1, Translocation protein SEC62

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Validation Data Gallery

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  WB analysis using 28693-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28693-1-AP (SEC62 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 28693-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed 48孔 HepG2 cells using SEC62 antibody (28693-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HT-29 cells, SW480 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28693-1-AP targets SEC62 in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEC62 fusion protein Ag29932
• Full Name: SEC62 homolog (S. cerevisiae)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: NM_003262
• Gene Symbol: SEC62
• Gene ID (NCBI): 7095
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEC62 is a integral endoplasmic reticulum (ER) membrane protein. SEC62 and SEC63 form a dimeric complex and play a central role in translocation of nascent and newly synthesized precursor polypeptides into the ER. SEC62 is associated with poor prognosis in a variety of tumors, such as prostate cancer, hepatocellular cancer, breast cancer , melanoma, colorectal cancer (PMID: 36262254).