SECISBP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SECISBP2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 12798-1-AP

Synonyms

Selenocysteine insertion sequence-binding protein 2, SECIS-binding protein 2, SECIS binding protein 2, SBP2

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Validation Data Gallery

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  WB analysis of HeLa using 12798-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12798-1-AP (SECISBP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12798-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12798-1-AP (SECISBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12798-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12798-1-AP (SECISBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12798-1-AP

  IP result of anti-SECISBP2 (IP:12798-1-AP, 3ug; Detection:12798-1-AP 1:600) with mouse testis tissue lysate 8000ug.

• 

  IF Staining of HepG2 using 12798-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SECISBP2 antibody (12798-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: HeLa cells, human kidney tissue, Jurkat cells
• Positive IP detected in: mouse testis tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Product Information

12798-1-AP targets SECISBP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SECISBP2 fusion protein Ag3541
• Full Name: SECIS binding protein 2
• Calculated Molecular Weight: 854 aa, 95 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC036109
• Gene Symbol: SECISBP2
• Gene ID (NCBI): 79048
• RRID: AB_2186555
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Selenium (Se) is an essential trace element required for the biosynthesis of selenoproteins, and selenocysteine insertion sequence (SECIS) binding protein 2 (SECISBP2, or SBP2) represents a key trans-acting factor for the cotranslational insertion of selenocysteine into selenoproteins. Defects in SBP2 are a cause of abnormal thyroid hormone metabolism (ATHYHM) associated with a reduction in type II iodothyronine deiodinase activity. Mutations in this gene have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism. Cells depleted of SBP2 have increased levels of ROS, which lead to cellular oxidative stress manifested as DNA lesions, stress granules, and lipid peroxidation, induction of caspase- and cytochrome c-dependent apoptosis, indicating that SBP2 is required for protection against ROS-induced cellular damage and cell survival.

Publications

Species	Application	Title
human	WB	Science; SELENOPROTEINS. CRL2 aids elimination of truncated selenoproteins produced by failed UGA/Sec decoding.; Authors - Hsiu-Chuan Lin
human	WB	Nat Chem Biol; Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability.; Authors - Zhipeng Li
human	WB	Nucleic Acids Res; Identification of a novel endogenous long non-coding RNA that inhibits selenoprotein P translation.; Authors - Yuichiro Mita
human	WB,IF	Free Radic Biol Med; Selective up-regulation of human selenoproteins in response to oxidative stress.; Authors - Touat-Hamici Zahia
mouse	WB	Endocrinology; Thyroid Hormone Metabolism Defects in a Mouse Model of SBP2 Deficiency.; Authors - Jiao Fu ; KO Validated
human	WB,IF	J Biol Chem; Interplay between Selenium Levels, Selenoprotein Expression and Replicative Senescence in WI-38 Human Fibroblasts.; Authors - Yona Legrain

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