FcZero-rAb™ CoraLite® Plus 488 Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocyte using CL488-FcA98084

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 488 Anti-Human SELPLG/CD162 Rabbit RecAb (CL488-FcA98084, Clone: 241297F10) or CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-FcA98136, Clone: 240953C9). Cells were not fixed.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

SELPLG/CD162 FcZero-rAb^TM Recombinant Antibody for FC

Cat No. CL488-FcA98084

Clone No.241297F10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

SELPLG, 241297F10, CD162, CLA, P-selectin glycoprotein ligand 1

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-FcA98084 targets SELPLG/CD162 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: selectin P ligand
• Calculated Molecular Weight: 412 aa, 43 kDa
• GenBank Accession Number: BC029782
• Gene Symbol: SELPLG
• Gene ID (NCBI): 6404
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

SELPLG (selectin P ligand), also known as CD162 or PSGL-1, is a 120 kDa type I transmembrane mucin-like glycoprotein. It is an adhesion molecule expressed on the surface of neutrophils, monocytes, and most lymphocytes as a homodimer of approximately 230-250 kDa (PMID: 7545173; 1378449; 9353350). SELPLG plays a critical role in the early stages of inflammation due to its ability to bind E-selectin (CD62E), L-selectin (CD62L), and P-selectin (CD62P) (PMID: 35597982).