SELS Polyclonal antibody

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• KD/KO Validated

SELS Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 15591-1-AP

Synonyms

SEPS1, SELENOS, Selenoprotein S, SBBI8, ADO15

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Validation Data Gallery

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  WB analysis of rat brain using 15591-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15591-1-AP (SELS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15591-1-AP

  WB result of SELS antibody (15591-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SELS transfected HeLa cells.

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  WB analysis of mouse brain using 15591-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15591-1-AP (SELS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15591-1-AP

  IP result of anti-SELS (IP:15591-1-AP, 3ug; Detection:15591-1-AP 1:500) with HepG2 cells lysate 600ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 15591-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15591-1-AP (SELS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 15591-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15591-1-AP (SELS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: rat brain tissue, HeLa cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 23 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

15591-1-AP targets SELS in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SELS fusion protein Ag7928
• Full Name: selenoprotein S
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC005840
• Gene Symbol: SELS
• Gene ID (NCBI): 55829
• RRID: AB_2263813
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SELS, also named as VIMP and SEPS1, plays an important role in the production of inflammatory cytokines and its expression is activated by endoplasmic reticulum (ER) stress. It probably acts by serving as a linker between DERL1 and VCP.

Publications

Species	Application	Title
human	WB	Sci Adv; Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.; Authors - Yuan Wang
pig	WB	J Anim Sci Biotechnol; Selenogenome and AMPK signal insight into the protective effect of dietary selenium on chronic heat stress-induced hepatic metabolic disorder in growing pigs.; Authors - Yan Liu
human	WB	Antioxidants (Basel); Dietary Marginal and Excess Selenium Increased Triglycerides Deposition, Induced Endoplasmic Reticulum Stress and Differentially Influenced Selenoproteins Expression in the Anterior and Middle Intestines of Yellow Catfish Pelteobagrus fulvidraco.; Authors - Dian-Guang Zhang
mouse	WB	Antioxidants (Basel); Reversal of Lipid Metabolism Dysregulation by Selenium and Folic Acid Co-Supplementation to Mitigate Pathology in Alzheimer's Disease.; Authors - Zhong-Hao Zhang
pig	WB	Antioxidants (Basel); Hydroxy Selenomethionine Improves Meat Quality through Optimal Skeletal Metabolism and Functions of Selenoproteins of Pigs under Chronic Heat Stress.; Authors - Yan Liu
pig	WB	Antioxidants (Basel); Hydroxy Selenomethionine Alleviates Hepatic Lipid Metabolism Disorder of Pigs Induced by Dietary Oxidative Stress via Relieving the Endoplasmic Reticulum Stress.; Authors - Jinzhong Jing