SEMA3B Recombinant antibody

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  WB analysis using 84080-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84080-3-RR (SEMA3B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of U-251 using 84080-3-RR

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SEMA3B antibody (84080-3-RR, Clone: 241171A7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84080-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84080-3-RR against Human SEMA3B were performed. The affinity constant is 8.53 nM.

SEMA3B Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84080-3-RR

Clone No.241171A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

241171A7, LUCA 1, SemA, Sema A(V), Sema V

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells, U-251 cells, SK-N-SH cells
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84080-3-RR targets SEMA3B in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SEMA3B fusion protein Ag25163
• Full Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC009113
• Gene Symbol: SEMA3B
• Gene ID (NCBI): 7869
• RRID: AB_3671648
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q13214
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEMA3B is a class 3 semaphorin/collapsin family member containing 749 highly conserved, NH2 terminal, semaphorin amino acids. SEMA3B is characterized as a strong suppressing factor of the proliferation of cancerous cells and also by its anti-angiogenic effect. SEMA3B has been found in lung cancer, ovarian cancer, hepatocellular carcinoma, and cholangiocarcinoma. SEMA3B is expressed abundantly but differentially in various neural and nonneural tissues. The calculated molecular weight of SEMA3B is 83 kDa (PMID: 30914894, PMID: 32410560).