SEMA3C Polyclonal antibody

• Featured Product
• KD/KO Validated

SEMA3C Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 19242-1-AP

Synonyms

Sema E, SEMA3C, SEMAE, Semaphorin 3C, Semaphorin E, SemE

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Validation Data Gallery

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  WB analysis of mouse heart using 19242-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19242-1-AP (SEMA3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 19242-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19242-1-AP (SEMA3C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below

Product Information

19242-1-AP targets SEMA3C in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEMA3C fusion protein Ag5480
• Full Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC030690
• Gene Symbol: SEMA3C
• Gene ID (NCBI): 10512
• RRID: AB_10858635
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEMA3C (Semaphorin 3C) is associated with tumor progression and poor prognosis across multiple tumor types, including pancreatic, gastric, prostate, and breast cancer, as well as glioma. It is worth noting that full-length SEMA3C has also been reported as a tumor suppressor factor by suppressing tumor lymphangiogenesis and metastasis (PMID: 31649890).

Publications

Species	Application	Title
human	WB	Front Oncol; SEMA3C Promotes Cervical Cancer Growth and Is Associated With Poor Prognosis.; Authors - Ruoyan Liu ; KD Validated
human	WB	Front Oncol; SEMA3C Supports Pancreatic Cancer Progression by Regulating the Autophagy Process and Tumor Immune Microenvironment.; Authors - Dalin Zhang ; KO Validated