Tested Applications
| Positive WB detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19242-1-AP targets SEMA3C in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5480 Product name: Recombinant human SEMA3C protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 34-380 aa of BC030690 Sequence: DELRETKTSEYFSLSHHPLDYRILLMDEDQDRIYVGSKDHILSLNINNISQEALSVFWPASTIKVEECKMAGKDPTHGCGNFVRVIQTFNRTHLYVCGSGAFSPVCTYLNRGRRSEDQVFMIDSKCESGKGRCSFNPNVNTVSVMINEELFSGMYIDFMGTDAAIFRSLTKRNAVRTDQHNSKWLSEPMFVDAHVIPDGTDPNDAKVYFFFKEKLTDNNRSTKQIHSMIARICPNDTGGLRSLVNKWTTFLKARLVCSVTDEDGPETHFDELEDVFLLETDNPRTTLVYGIFTTSSSVFKGSAVCVYHLSDIQTVFNGPFAHKEGPNHQLISYQGRIPYPRPGTCPG Predict reactive species |
| Full Name | sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C |
| Calculated Molecular Weight | 85 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC030690 |
| Gene Symbol | SEMA3C |
| Gene ID (NCBI) | 10512 |
| RRID | AB_10858635 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99985 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SEMA3C (Semaphorin 3C) is associated with tumor progression and poor prognosis across multiple tumor types, including pancreatic, gastric, prostate, and breast cancer, as well as glioma. It is worth noting that full-length SEMA3C has also been reported as a tumor suppressor factor by suppressing tumor lymphangiogenesis and metastasis (PMID: 31649890).
Publications
What published studies show
The SEMA3C Polyclonal antibody (Cat# 19242-1-AP) has 3 citations. These appear in EMBO Molecular Medicine and Frontiers in Oncology. The research fields covered include prostate cancer (PlexinD1 as a therapeutic target), pancreatic cancer (SEMA3C regulating autophagy and tumor immune microenvironment), and cervical cancer (SEMA3C promoting tumor growth and associated with poor prognosis). All citations involve human samples, with applications in Western blot and immunofluorescence.
| Species | Application | Title |
|---|---|---|
Front Oncol SEMA3C Promotes Cervical Cancer Growth and Is Associated With Poor Prognosis.
| ||
Front Oncol SEMA3C Supports Pancreatic Cancer Progression by Regulating the Autophagy Process and Tumor Immune Microenvironment.
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