SEMA4A Polyclonal antibody

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• KD/KO Validated

SEMA4A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27359-1-AP

Synonyms

CORD10, RP35, Sema B, SEMA4A, SEMAB, Semaphorin 4A, Semaphorin B, SEMB

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Validation Data Gallery

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  WB analysis using 27359-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27359-1-AP (SEMA4A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27359-1-AP

  WB result of SEMA4A antibody (27359-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEMA4A transfected HeLa cells.

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  IHC staining of human breast cancer using 27359-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27359-1-AP (SEMA4A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27359-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27359-1-AP (SEMA4A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 27359-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27359-1-AP (SEMA4A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 27359-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27359-1-AP (SEMA4A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, PC-3 cells
• Positive IHC detected in: human breast cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

27359-1-AP targets SEMA4A in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEMA4A fusion protein Ag26023
• Full Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A
• Calculated Molecular Weight: 761 aa, 84 kDa
• Observed Molecular Weight: 120-150 kDa
• GenBank Accession Number: BC020974
• Gene Symbol: SEMA4A
• Gene ID (NCBI): 64218
• RRID: AB_2880854
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sema4A, the membrane-bound class 4 semaphorin, belongs to semaphorins superfamily. Sema4A funtions as potent axonal repellants in the nervous system, and is involved in vascular development. Two species of Sema4A with molecular weights of about 150 and 120 kDa were observed. The antibody recognizes the Sema4A protein, which is of appropriate molecular size (~120 kDa) for the glycosylated protein(PMID: 26296689/17318185/26024919).

Publications

Species	Application	Title
human	WB	J Cancer; SEMA4A promotes prostate cancer invasion: involvement of tumor microenvironment; Authors - Xiao Liu