APC Anti-Human SEMA7A Rabbit Recombinant Antibody

• Home
• Products
• Primary Antibodies
• Flow Cytometry Antibodies
• APC Anti-Human SEMA7A Rabbit Recombinant Antibody (APC-98324-3)

SEMA7A Uni-rAb^® Recombinant Antibody for FC

Cat No. APC-98324-3

Clone No.241814B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD108, CDw108, John-Milton-Hargen human blood group Ag, Sema K1, Sema L

• 

  FC experiment of human PBMCs using APC-98324-3

  1x10^6 untreated or PHA-treated human PBMCs were surface stained with 5 ul APC Anti-Human SEMA7A Rabbit RecAb (APC-98324-3, Clone: 241814B12). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using APC-98324-3

  1x10^6 PHA-treated human PBMCs were surface stained with 5 ul APC Anti-Human SEMA7A Rabbit RecAb (APC-98324-3, Clone: 241814B12) (red) or APC Rabbit IgG Isotype Control Recombinant Antibody (APC-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul APC Anti-Human SEMA7A Rabbit RecAb (APC-98324-3, Clone: 241814B12) (black, dashed). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive FC detected in: PHA treated human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

APC-98324-3 targets SEMA7A in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2005

  Product name: Recombinant Human SEMA7A protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 45-648 aa of NM_003612.5

  Sequence: QGHLRSGPRIFAVWKGHVGQDRVDFGQTEPHTVLFHEPGSSSVWVGGRGKVYLFDFPEGKNASVRTVNIGSTKGSCLDKRDCENYITLLERRSEGLLACGTNARHPSCWNLVNGTVVPLGEMRGYAPFSPDENSLVLFEGDEVYSTIRKQEYNGKIPRFRRIRGESELYTSDTVMQNPQFIKATIVHQDQAYDDKIYYFFREDNPDKNPEAPLNVSRVAQLCRGDQGGESSLSVSKWNTFLKAMLVCSDAATNKNFNRLQDVFLLPDPSGQWRDTRVYGVFSNPWNYSAVCVYSLGDIDKVFRTSSLKGYHSSLPNPRPGKCLPDQQPIPTETFQVADRHPEVAQRVEPMGPLKTPLFHSKYHYQKVAVHRMQASHGETFHVLYLTTDRGTIHKVVEPGEQEHSFAFNIMEIQPFRRAAAIQTMSLDAERRKLYVSSQWEVSQVPLDLCEVYGGGCHGCLMSRDPYCGWDQGRCISIYSSERSVLQSINPAEPHKECPNPKPDKAPLQKVSLAPNSRYYLSCPMESRHATYSWRHKENVEQSCEPGHQSPNCILFIENLTAQQYGHYFCEAQEGSYFREAQHWQLLPEDGIMAEHLLGHACALA

• Full Name: semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group)
• Calculated Molecular Weight: 75kDa
• GenBank Accession Number: NM_003612.5
• Gene Symbol: SEMA7A
• Gene ID (NCBI): 8482
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75326-1
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

SEMA7A, also named CD108 and SEMAL, belongs to the semaphorin family. SEMA7A is the only membrane-associated glycosylphosphatidylinositol (GPI)-linked semaphorin and plays a role in the regulation of neuronal axon outgrowth. In addition to its role in guiding axon pathfinding during neuronal development, SEMA7A has diverse functions in morphogenesis and immune cell control and regulates T cell responses via the α1β1 integrin receptor.