|Positive WB detected in||HepG2 cells, HeLa cells, HL-60 cells, Jurkat cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human ovary tissue, human liver tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 25349-1-AP is SENP1 Fusion Protein expressed in E. coli.
|Host / Isotype||Rabbit / IgG|
|Immunogen||SENP1 fusion protein Ag18028|
|Full Name||SUMO1/sentrin specific peptidase 1|
|Calculated molecular weight||644 aa, 73 kDa|
|Observed molecular weight||73 kDa|
|GenBank accession number||BC045639|
|Gene ID (NCBI)||29843|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Chun (Verified Customer) (09-07-2020)
This is a great antibody for immunoblotting.