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  • KD/KO Validated

SENP1 Polyclonal antibody

SENP1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 25349-1-AP

Synonyms

SENP1, Sentrin specific protease 1, SuPr 2



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HL-60 cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman ovary tissue, human liver tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25349-1-AP is SENP1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SENP1 fusion protein Ag18028
Full Name SUMO1/sentrin specific peptidase 1
Calculated molecular weight 644 aa, 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC045639
Gene symbol SENP1
Gene ID (NCBI) 29843
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SENP1 antibody 25349-1-APDownload protocol
IHC protocol for SENP1 antibody 25349-1-APDownload protocol
IP protocol for SENP1 antibody 25349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biol Sci

SENP1 Interacts with HIF1α to Regulate Glycolysis of Prostatic Carcinoma Cells.

Authors - Chunyang Wang
  • KD Validated
humanWB

J Pharmacol Sci

Momordin Ic induces G0/1 phase arrest and apoptosis in colon cancer cells by suppressing SENP1/c-MYC signaling pathway.

Authors - Fang Xianjun
  • KD Validated
humanWB, IHC

Cancer Med

Long noncoding RNA MCM3AP-AS1 enhances cell proliferation and metastasis in colorectal cancer by regulating miR-193a-5p/SENP1.

Authors - Mingyue Zhou
ratWB

Cell Prolif

SCARF1 promotes M2 polarization of Kupffer cells via calcium-dependent PI3K-AKT-STAT3 signalling to improve liver transplantation.

Authors - Xue-Song Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chun (Verified Customer) (09-07-2020)

This is a great antibody for immunoblotting.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa