Tested Applications
| Positive WB detected in | mouse brain tissue, A375 cells, rat brain tissue, mouse cerebellum tissue |
| Positive IP detected in | A375 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12476-1-AP targets Septin 4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3185 Product name: Recombinant human SEPT4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 113-459 aa of BC018056 Sequence: NQVHRKSVKKGFDFTLMVAGESGLGKSTLVNSLFLTDLYRDRKLLGAEERIMQTVEITKHAVDIEEKGVRLRLTIVDTPGFGDAVNNTECWKPVAEYIDQQFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPLDVEFMKALHQRVNIVPILAKADTLTPPEVDHKKRKIREEIEHFGIKIYQFPDCDSDEDEDFKLQDQALKESIPFAVIGSNTVVEARGRRVRGRLYPWGIVEVENPGHCDFVKLRTMLVRTHMQDLKDVTRETHYENYRAQCIQSMTRLVVKERNRNKLTRESGTDFPIPAVPPGTDPETEKLIREKDEELRRMQEMLHKIQKQMKENY Predict reactive species |
| Full Name | septin 4 |
| Calculated Molecular Weight | 459 aa, 53 kDa |
| Observed Molecular Weight | 54 kDa, 52 kDa, 48 kDa, 44 kDa |
| GenBank Accession Number | BC018056 |
| Gene Symbol | SEPT4 |
| Gene ID (NCBI) | 5414 |
| RRID | AB_2187151 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43236 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Spetin 4 is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Spetin 4 was widely expressed in brain and heart, also expressed in colorectal cancers and malignant melanomas. This protein has been implicated in diverse cellular functions, including cytokinesis, apoptosis, and tumor suppression(PMID:15737931). There are several isoforms of SEPTIN4, four of which were detected in the mouse brain and mouse cerebellum, 54 kDa, 52 kDa, 48 kDa and 44 kDa respectively(PMID: 23938054; PMID: 26657011).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Septin 4 antibody 12476-1-AP | Download protocol |
| IHC protocol for Septin 4 antibody 12476-1-AP | Download protocol |
| IP protocol for Septin 4 antibody 12476-1-AP | Download protocol |
| WB protocol for Septin 4 antibody 12476-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Septin 4 Polyclonal antibody (12476-1-AP) has 10 citations published in journals including Nature Communications, Cell Death & Disease, EMBO Reports, International Journal of Biological Sciences, Biomedical Pharmacotherapy, Frontiers in Endocrinology, Bioorganic Chemistry, Asian Journal of Andrology, Biochemical and Biophysical Research Communications, and bioRxiv. Research fields span sperm biology and male infertility, cardiovascular and vascular biology, cancer metastasis, and oxidative stress-mediated cell injury.
| Species | Application | Title |
|---|---|---|
Nat Commun The TEX44-CPT1B axis regulates mitochondrial sheath assembly and fatty acid oxidation in sperm | ||
Cell Death Dis TMEM232 is required for the formation of sperm flagellum and male fertility in mice | ||
Int J Biol Sci Septin4 Prevents PDGF-BB-induced HAVSMC Phenotypic Transformation, Proliferation and Migration by Promoting SIRT1-STAT3 Deacetylation and Dephosphorylation.
| ||
Biomed Pharmacother Atorvastatin rescues vascular endothelial injury in hypertension by WWP2-mediated ubiquitination and degradation of ATP5A | ||
EMBO Rep Basal body docking failure triggers centriole clustering and elimination in mammalian spermatogenesis. | ||
Front Endocrinol (Lausanne) A novel mutation in CFAP47 causes male infertility due to multiple morphological abnormalities of the sperm flagella |
Reviews
What customers say
The single review for this product is highly positive, with a 5-star rating. The reviewer used it for immunofluorescence on testis tissue at a 1:200 dilution and reported that Septin 4 stained the annulus of sperm beautifully, with very little background and a sharp, clear signal. Overall, the feedback highlights excellent specificity and low background in IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mohammed (Verified Customer) (08-19-2020) | Septin 4 stained the annulus of sperm very beautifully. Very little background and a nice sharp stain.
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