Septin 4 Polyclonal antibody

• Featured Product
• KD/KO Validated

Septin 4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 12476-1-AP

Synonyms

ARTS, BRADEION, Bradeion beta, Brain protein H5, CE5B3, CE5B3 beta, Cerebral protein 7, H5, hCDCREL 2, hucep 7, MART, Peanut like protein 2, PNUTL2, SEP4, SEPT4, septin 4, Septin4

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Validation Data Gallery

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  WB analysis using 12476-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12476-1-AP (Septin 4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12476-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12476-1-AP (Septin 4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A375 using 12476-1-AP

  IP result of anti-Septin 4 (IP:12476-1-AP, 4ug; Detection:12476-1-AP 1:300) with A375 cells lysate 2200ug.

• 

  IHC staining of mouse brain using 12476-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12476-1-AP (Septin 4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12476-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12476-1-AP (Septin 4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of hTERT-RPE1 using 12476-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using Septin 4 antibody (12476-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, A375 cells, rat brain tissue, mouse cerebellum tissue
• Positive IP detected in: A375 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

12476-1-AP targets Septin 4 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse , rat samples.

• Tested Reactivity: human, mouse , rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Septin 4 fusion protein Ag3185
• Full Name: septin 4
• Calculated Molecular Weight: 459 aa, 53 kDa
• Observed Molecular Weight: 54 kDa, 52 kDa, 48 kDa, 44 kDa
• GenBank Accession Number: BC018056
• Gene Symbol: SEPT4
• Gene ID (NCBI): 5414
• RRID: AB_2187151
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Spetin 4 is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Spetin 4 was widely expressed in brain and heart, also expressed in colorectal cancers and malignant melanomas. This protein has been implicated in diverse cellular functions, including cytokinesis, apoptosis, and tumor suppression(PMID:15737931). There are several isoforms of SEPTIN4, four of which were detected in the mouse brain and mouse cerebellum, 54 kDa, 52 kDa, 48 kDa and 44 kDa respectively(PMID: 23938054; PMID: 26657011).

Publications

Species	Application	Title
human	WB	Int J Biol Sci; Septin4 Prevents PDGF-BB-induced HAVSMC Phenotypic Transformation, Proliferation and Migration by Promoting SIRT1-STAT3 Deacetylation and Dephosphorylation.; Authors - Naijin Zhang ; KD Validated
human	WB,IF,IP	Biochem Biophys Res Commun; Septin4 as a novel binding partner of PARP1 contributes to oxidative stress induced human umbilical vein endothelial cells injure.; Authors - Naijin Zhang ; KD Validated
human,mouse	IF,CoIP	Front Endocrinol (Lausanne); A novel mutation in CFAP47 causes male infertility due to multiple morphological abnormalities of the sperm flagella; Authors - Mohan Liu
mouse	WB	Biomed Pharmacother; Atorvastatin rescues vascular endothelial injury in hypertension by WWP2-mediated ubiquitination and degradation of ATP5A; Authors - Zeyu Yin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mohammed (Verified Customer) (08-19-2020)

Septin 4 stained the annulus of sperm very beautifully. Very little background and a nice sharp stain.

• Applications: Immunofluorescence
• Primary Antibody Dilution: (1:200)
• Cell Tissue Type: Testis