SEPT4 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82284-1-RR (same clone as 82284-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82284-1-RR (SEPT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82284-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82284-1-RR (same clone as 82284-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82284-1-RR (SEPT4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82284-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82284-1-RR (same clone as 82284-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82284-1-RR (SEPT4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82284-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82284-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82284-1-RR against Human SEPT4 were performed. The affinity constant is 1.38 nM.

SEPT4 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 82284-1-PBS

Clone No.5O8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

hCDCREL-2, hCDCREL2, Cell division control-related protein 2, C17orf47, BRADEION

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Product Information

82284-1-PBS targets SEPT4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3185

  Product name: Recombinant human SEPT4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 113-459 aa of BC018056

  Sequence: NQVHRKSVKKGFDFTLMVAGESGLGKSTLVNSLFLTDLYRDRKLLGAEERIMQTVEITKHAVDIEEKGVRLRLTIVDTPGFGDAVNNTECWKPVAEYIDQQFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPLDVEFMKALHQRVNIVPILAKADTLTPPEVDHKKRKIREEIEHFGIKIYQFPDCDSDEDEDFKLQDQALKESIPFAVIGSNTVVEARGRRVRGRLYPWGIVEVENPGHCDFVKLRTMLVRTHMQDLKDVTRETHYENYRAQCIQSMTRLVVKERNRNKLTRESGTDFPIPAVPPGTDPETEKLIREKDEELRRMQEMLHKIQKQMKENY

• Full Name: septin 4
• Calculated Molecular Weight: 459 aa, 53 kDa
• Observed Molecular Weight: 38-55 kDa
• GenBank Accession Number: BC018056
• Gene Symbol: SEPT4
• Gene ID (NCBI): 5414
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43236
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Spetin 4 is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Spetin 4 was widely expressed in brain and heart, also expressed in colorectal cancers and malignant melanomas. This protein has been implicated in diverse cellular functions, including cytokinesis, apoptosis, and tumor suppression(PMID:15737931). There are several isoforms of SEPTIN4, four of which were detected in the mouse brain and mouse cerebellum, 54 kDa, 52 kDa, 48 kDa and 44 kDa respectively(PMID: 23938054; PMID: 26657011).