SEPT4 Recombinant antibody

SEPT4 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5O8

Cat no : 82284-1-RR

Synonyms

ARTS, BRADEION, Bradeion beta, Brain protein H5, CE5B3, CE5B3 beta, Cerebral protein 7, H5, hCDCREL 2, hucep 7, MART, Peanut like protein 2, PNUTL2, SEP4, SEPT4, septin 4

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Validation Data Gallery

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  WB analysis using 82284-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82284-1-RR (SEPT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 82284-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82284-1-RR (SEPT4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 82284-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82284-1-RR (SEPT4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82284-1-RR targets SEPT4 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SEPT4 fusion protein Ag3185
• Full Name: septin 4
• Calculated Molecular Weight: 459 aa, 53 kDa
• Observed Molecular Weight: 38-55 kDa
• GenBank Accession Number: BC018056
• Gene Symbol: SEPT4
• Gene ID (NCBI): 5414
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Spetin 4 is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Spetin 4 was widely expressed in brain and heart, also expressed in colorectal cancers and malignant melanomas. This protein has been implicated in diverse cellular functions, including cytokinesis, apoptosis, and tumor suppression(PMID:15737931). There are several isoforms of SEPTIN4, four of which were detected in the mouse brain and mouse cerebellum, 54 kDa, 52 kDa, 48 kDa and 44 kDa respectively(PMID: 23938054; PMID: 26657011).