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  • KD/KO Validated

Septin 9 Polyclonal antibody

Septin 9 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10769-1-AP

Synonyms

SEPT9, Septin-9, MSF, MLL septin-like fusion protein MSF-A, MLL septin-like fusion protein



Tested Applications

Positive WB detected inC6 cells, HeLa cells, mouse spleen tissue, mouse brain tissue, mouse thymus tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue, human colon tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, hTERT-RPE cells, HepG2 cells, A431 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10769-1-AP targets Septin 9 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine, drosophila
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Septin 9 fusion protein Ag1218
Full Name septin 9
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 65 kDa, 38-40 kDa
GenBank Accession NumberBC021192
Gene Symbol SEPT9
Gene ID (NCBI) 10801
RRIDAB_2185012
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).

Protocols

Product Specific Protocols
WB protocol for Septin 9 antibody 10769-1-APDownload protocol
IHC protocol for Septin 9 antibody 10769-1-APDownload protocol
IF protocol for Septin 9 antibody 10769-1-APDownload protocol
IP protocol for Septin 9 antibody 10769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Dev Cell

Polarity of Neuronal Membrane Traffic Requires Sorting of Kinesin Motor Cargo during Entry into Dendrites by a Microtubule-Associated Septin.

Authors - Eva P Karasmanis
  • KD Validated
canineIF

Curr Biol

A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.

Authors - Eva P Karasmanis
  • KD Validated
humanWB,IF

Curr Biol

Cdc42 and its BORG2 and BORG3 effectors control the subcellular localization of septins between actin stress fibers and microtubules.

Authors - Joëlle Salameh
humanWB, IF

J Cell Biol

A septin GTPase scaffold of dynein-dynactin motors triggers retrograde lysosome transport.

Authors - Ilona A Kesisova
humanIF

J Cell Biol

Septins suppress the release of vaccinia virus from infected cells.

Authors - Julia Pfanzelter
mouseWB

PLoS Genet

Genetic deletion of SEPT7 reveals a cell type-specific role of septins in microtubule destabilization for the completion of cytokinesis.

Authors - Manoj B Menon