Septin 9 Polyclonal antibody

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• Septin 9 Polyclonal antibody (10769-1-AP)

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• KD/KO Validated

Septin 9 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10769-1-AP Copied!

Publications (38)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

SEPT9, KIAA0991, MLL septin-like fusion protein, MLL septin-like fusion protein MSF-A, MSF

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  WB analysis using 10769-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10769-1-AP (Septin 9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 10769-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10769-1-AP (Septin 9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 10769-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10769-1-AP (Septin 9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse spleen using 10769-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10769-1-AP (Septin 9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 10769-1-AP

  IP result of anti-Septin 9 (IP:10769-1-AP, 4ug; Detection:10769-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 10769-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10769-1-AP (Septin 9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 10769-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10769-1-AP (Septin 9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 10769-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10769-1-AP (Septin 9 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 10769-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10769-1-AP (Septin 9 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 10769-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10769-1-AP (Septin 9 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of hTERT-RPE cells using 10769-1-AP

  IF result of anti-Septin 9 (10769-1-AP, 1:200) with hTERT-RPE cell by Moshe Kim (laboratory of Dr. William S Trimble, University of Toronto). Green: SEPT9. Blue: DNA. Fixation in methanol.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 10769-1-AP

  Immunofluorescent analysis of HepG2 cells, using Septin 9 antibody 10769-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 10769-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Septin 9 antibody (10769-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 10769-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Septin 9 antibody (10769-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HepG2 using 10769-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Septin 9 (10769-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: C6 cells, HeLa cells, mouse spleen tissue, mouse brain tissue, mouse thymus tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human pancreas cancer tissue, human colon tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, hTERT-RPE cells, HepG2 cells, A431 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10769-1-AP targets Septin 9 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1218

  Product name: Recombinant human SEPT9 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 250-568 aa of BC021192

  Sequence: RNEKAPVDFGYVGIDSILEQMRRKAMKQGFEFNIMVVGQSGLGKSTLINTLFKSKISRKSVQPTSEERIPKTIEIKSITHDIEEKGVRMKLTVIDTPGFGDHINNENCWQPIMKFINDQYEKYLQEEVNINRKKRIPDTRVHCCLYFIPATGHSLRPLDIEFMKRLSKVVNIVPVIAKADTLTLEERVHFKQRITADLLSNGIDVYPQKEFDEDSEDRLVNEKFREMIPFAVVGSDHEYQVNGKRILGRKTKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDITSSIHFEAYRVKRLNEGSSAMANGVEEKEPEAPEM

• Full Name: septin 9
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 65 kDa, 38-40 kDa
• GenBank Accession Number: BC021192
• Gene Symbol: SEPT9
• Gene ID (NCBI): 10801
• RRID: AB_2185012
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UHD8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).

Publications

What published studies show

Anti-SEPT9 antibody (Cat# 10769-1-AP) has 38 citations across 25 journals, including Nature Communications, Current Biology, Journal of Cell Biology, Oncogene, Developmental Cell, and PLoS Genetics. Research fields span septin cytoskeleton biology, cytokinesis, microtubule dynamics, cancer (breast, colorectal, cervical), neuroscience, diabetes, Huntington's disease, cardiology, virology, and proteomics.

Automatically generated from the 38 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	IF	Nat Commun; Quantitative determination of the spatial distribution of components in single cells with CellDetail; Authors - Tanja Schuster
human	WB	Pharmacol Res; BET degrader exhibits lower antiproliferative activity than its inhibitor via EGR1 recruiting septins to promote E2F1-3 transcription in triple-negative breast cancer; Authors - Nan Liu ; KD Validated
human	WB	Cell Death Dis; Septin filament coalignment with microtubules depends on SEPT9_i1 and tubulin polyglutamylation, and is an early feature of acquired cell resistance to paclitaxel.; Authors - Benjamin Targa
rat	IF	Dev Cell; Polarity of Neuronal Membrane Traffic Requires Sorting of Kinesin Motor Cargo during Entry into Dendrites by a Microtubule-Associated Septin.; Authors - Eva P Karasmanis ; KD Validated
human	WB,IF	Oncogene; Septin 9 isoforms promote tumorigenesis in mammary epithelial cells by increasing migration and ECM degradation through metalloproteinase secretion at focal adhesions.; Authors - Jenna Marcus
human	WB,IF	Curr Biol; Cdc42 and its BORG2 and BORG3 effectors control the subcellular localization of septins between actin stress fibers and microtubules.; Authors - Joëlle Salameh