SERCA2/ATP2A2 Recombinant antibody

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  WB analysis using 86050-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86050-1-RR (SERCA2,ATP2A2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 86050-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 86050-1-RR (SERCA2/ATP2A2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using 86050-1-RR

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using SERCA2/ATP2A2 antibody (86050-1-RR, Clone: 250667D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86050-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86050-1-RR against Human SERCA2/ATP2A2 were performed. The affinity constant is below 1 pM.

SERCA2/ATP2A2 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 86050-1-RR

Clone No.250667D7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ATP2A2, SERCA2, SERCA2b, ATP2A 2, ATP2B

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Tested Applications

• Positive WB detected in: 37°C incubated mouse heart tissue, 37℃ incubated mouse heart, rat heart, 37℃ incubated rat heart
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86050-1-RR targets SERCA2/ATP2A2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SERCA2/ATP2A2 fusion protein Ag26177
• Full Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
• Calculated Molecular Weight: 1042 aa, 115 kDa
• Observed Molecular Weight: 110-115 kDa
• GenBank Accession Number: BC035588
• Gene Symbol: SERCA2
• Gene ID (NCBI): 488
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16615
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.