SERINC3 Polyclonal antibody

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  WB analysis of mouse pancreas using 20267-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20267-1-AP (SERINC3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 20267-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20267-1-AP (SERINC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 20267-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20267-1-AP (SERINC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SERINC3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20267-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

TDE1, TDE, SERINC 3, SBBI99, DIFF33

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Tested Applications

• Positive WB detected in: mouse pancreas tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20267-1-AP targets SERINC3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: serine incorporator 3
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: NM_006811
• Gene Symbol: SERINC3
• Gene ID (NCBI): 10955
• RRID: AB_10693614
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13530
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERINC, also named as DIFF33 and TDE1, belongs to the TDE1 family. SERINC3 may be involved in cellular transformation. The antibody is specific to SERINC3.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (01-25-2019)

By WB, I saw a band around 42 kD, but the expected band is around 53 kD. The 42 kD band did not disappear when I knock it down with smart-pool RNAs.By IF, I didn't see its Golgi localisation, which is shown in literature.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MEF