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SERINC3 Polyclonal antibody

SERINC3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20267-1-AP

Synonyms

TDE1, TDE, SERINC 3, SBBI99, DIFF33

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using SERINC3 Polyclonal antibody (20267-1-AP)

    WB analysis of mouse pancreas using 20267-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20267-1-AP (SERINC3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SERINC3 Polyclonal antibody (20267-1-AP)

    IHC staining of human kidney using 20267-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20267-1-AP (SERINC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SERINC3 Polyclonal antibody (20267-1-AP)

    IHC staining of human kidney using 20267-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20267-1-AP (SERINC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20267-1-AP targets SERINC3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name serine incorporator 3
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberNM_006811
Gene Symbol SERINC3
Gene ID (NCBI) 10955
RRIDAB_10693614
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERINC, also named as DIFF33 and TDE1, belongs to the TDE1 family. SERINC3 may be involved in cellular transformation. The antibody is specific to SERINC3.

Protocols

Product Specific Protocols
WB protocol for SERINC3 antibody 20267-1-APDownload protocol
IHC protocol for SERINC3 antibody 20267-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Boyan (Verified Customer) (01-25-2019)

By WB, I saw a band around 42 kD, but the expected band is around 53 kD. The 42 kD band did not disappear when I knock it down with smart-pool RNAs.By IF, I didn't see its Golgi localisation, which is shown in literature.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: MEF