Alpha Antichymotrypsin Monoclonal antibody

Alpha Antichymotrypsin Monoclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF, FC

Conjugate

Unconjugated

CloneNo.

3F2F10

Cat no : 66078-1-Ig

Synonyms

A1 Antichymotrypsin, A-1-Antichymotrypsin, AACT, ACT, Alpha 1 antichymotrypsin, GIG24, GIG25, Serpin A3, SERPINA3



Tested Applications

Positive WB detected inhuman plasma tissue, human colon tissue
Positive IHC detected inhuman tonsillitis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66078-1-Ig is Alpha Antichymotrypsin Fusion Protein expressed in E. coli.

Tested Reactivity human, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Alpha Antichymotrypsin fusion protein Ag2830
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
Calculated molecular weight 448 aa, 48 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC010530
Gene symbol SERPINA3
Gene ID (NCBI) 12
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson's disease (PD), Alzheimer's disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy.

Protocols

Product Specific Protocols
WB protocol for Alpha Antichymotrypsin antibody 66078-1-IgDownload protocol
IHC protocol for Alpha Antichymotrypsin antibody 66078-1-IgDownload protocol
IF protocol for Alpha Antichymotrypsin antibody 66078-1-IgDownload protocol
FC protocol for Alpha Antichymotrypsin antibody 66078-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Br J Cancer

Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.

Authors - Yanxia Jin
humanWB,IHC

Breast Cancer

Overexpression of SERPINA3 promotes tumor invasion and migration, epithelial-mesenchymal-transition in triple-negative breast cancer cells.

Authors - Yingzi Zhang