SERPINA7/ TBG Monoclonal antibody

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  WB analysis of human plasma using 66454-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66454-1-Ig (SERPINA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 66454-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66454-1-Ig (SERPINA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66454-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66454-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 66454-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66454-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 66454-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERPINA7 antibody (66454-1-Ig, Clone: 1B11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 66454-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERPINA7 antibody (66454-1-Ig, Clone: 1B11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

SERPINA7/ TBG Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 66454-1-Ig

Clone No.1B11B10

Publications(1)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Synonyms

SERPINA7, 1B11B10, Serpin A7, SERPINA 7

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Tested Applications

• Positive WB detected in: human plasma tissue, human testis tissue
• Positive IHC detected in: human liver cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

66454-1-Ig targets SERPINA7/ TBG in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9823

  Product name: Recombinant human SERPINA7 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 38-415 aa of BC020747

  Sequence: TLYKMSSINADFAFNLYRRFTVETPDKNIFFSPVSISAALVMLSFGACCSTQTEIVETLGFNLTDTPMVEIQHGFQHLICSLNFPKKELELQIGNALFIGKHLKPLAKFLNDVKTLYETEVFSTDFSNISAAKQEINSHVEMQTKGKVVGLIQDLKPNTIMVLVNYIHFKAQWANPFDPSKTEDSSSFLIDKTTTVQVPMMHQMEQYYHLVDMELNCTVLQMDYSKNALALFVLPKEGQMESVEAAMSSKTLKKWNRLLQKGWVDLFVPKFSISATYDLGATLLKMGIQHAYSENADFSGLTEDNGLKLSNAAHKAVLHIGEKGTEAAAVPEVELSDQPENTFLHPIIQIDRSFMLLILERSTRSILFLGKVVNPTEA

• Full Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
• Calculated Molecular Weight: 415 aa, 46 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC020747
• Gene Symbol: SERPINA7
• Gene ID (NCBI): 6906
• RRID: AB_2881823
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05543
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Heliyon; Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study; Authors - Lingyan Chen