SERPINB13 Polyclonal antibody

SERPINB13 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 18045-1-AP

Synonyms

Serpin B13, Peptidase inhibitor 13, Hurpin, HUR7, headpin

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Validation Data Gallery

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  IHC staining of human oesophagus cancer using 18045-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18045-1-AP (SERPINB13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 18045-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18045-1-AP (SERPINB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 18045-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18045-1-AP (SERPINB13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 18045-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18045-1-AP (SERPINB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 18045-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18045-1-AP (SERPINB13 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 18045-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18045-1-AP (SERPINB13 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 18045-1-AP

  Immunofluorescent analysis of HepG2 cells, using SERPINB13 antibody 18045-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 18045-1-AP

  Immunofluorescent analysis of Hela cells, using SERPINB13 antibody 18045-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 18045-1-AP

  mmunofluorescent analysis of Hela cells, using SERPINB13 antibody 18045-1-AP at 1:25 dilution and FITC-Donkey anti-Rabbit IgG (green).

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  FC experiment of HepG2 using 18045-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug SERPINB13 Polyclonal antibody (18045-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IHC detected in: human oesophagus cancer tissue, mouse skin tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, Hela cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18045-1-AP targets SERPINB13 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SERPINB13 fusion protein Ag12686
• Full Name: serpin peptidase inhibitor, clade B (ovalbumin), member 13
• Calculated Molecular Weight: 391 aa, 44 kDa
• GenBank Accession Number: BC101821
• Gene Symbol: SERPINB13
• Gene ID (NCBI): 5275
• RRID: AB_2186160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.