SERPINB2 Polyclonal antibody

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  WB analysis using 16035-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16035-1-AP (SERPINB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 16035-1-AP

  IP result of anti-SERPINB2 (IP:16035-1-AP, 4ug; Detection:16035-1-AP 1:300) with Jurkat cells lysate 3600ug.

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  IHC staining of mouse brain using 16035-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16035-1-AP (SERPINB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16035-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16035-1-AP (SERPINB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SERPINB2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 16035-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

HsT1201, Monocyte Arg serpin, PAI, PAI 2, PAI2, PAI-2, PLANH2, Serpin B2, SERPIN2, SERPINB2, Urokinase inhibitor

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Tested Applications

• Positive WB detected in: A431 cells, Jurkat cells
• Positive IP detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 3 publications below

Product Information

16035-1-AP targets SERPINB2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8906

  Product name: Recombinant human SERPINB2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-415 aa of BC012609

  Sequence: MEDLCVANTLFALNLFKHLAKASPTQNLFLSPWSISSTMAMVYMGSRGSTEDQMAKVLQFNEVGANAVTPMTPENFTSCGFMQQIQKGSYPDAILQAQAADKIHSSFRSLSSAINASTGNYLLESVNKLFGEKSASFREEYIRLCQKYYSSEPQAVDFLECAEEARKKIYSWVKTQTKGKIPNLLPEGSVDGDTRMVLVNAVYFKGKWKTPFEKKLNGLYPFRVNSAQRTPVQMMYLREKLNIGYIEDLKAQILELPYAGDVSMFLLLPDEIADVSTGLELLESEITYDKLNKWTSKDKMAEDEVEVYIPQFKLEEHYELRSILRSMGMEDAFNKGRANFSGMSERNDLFLSEVFHQAMVDVNEEGTEAAAGTGGVMTGRTGHGGPQFVADHPFLFLIMHKITNCILFFGRFSSP

• Full Name: serpin peptidase inhibitor, clade B (ovalbumin), member 2
• Calculated Molecular Weight: 415 aa, 47 kDa
• Observed Molecular Weight: 45-47 kDa
• GenBank Accession Number: BC012609
• Gene Symbol: SERPINB2
• Gene ID (NCBI): 5055
• RRID: AB_2186180
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P05120
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Oncotarget; Exposure to airborne PM2.5 suppresses microRNA expression and deregulates target oncogenes that cause neoplastic transformation in NIH3T3 cells.; Authors - Chunling Liu
	IHC	PLoS One; Plasminogen Activator Inhibitor-2 Plays a Leading Prognostic Role among Protease Families in Non-Small Cell Lung Cancer.; Authors - Chia-Yi Su
human	WB	J Proteomics; Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection.; Authors - Dan Zhang
human	IHC,WB	Mol Cancer; circPSD3 is a promising inhibitor of uPA system to inhibit vascular invasion and metastasis in hepatocellular carcinoma; Authors - Liangliang Xu
human	WB	Carcinogenesis; Capsaicin enhances cisplatin-induced anti-metastasis of nasopharyngeal carcinoma by inhibiting EMT and ERK signaling via SERPINB2; Authors - Yafei Xu
human	WB	Aging (Albany NY); Unveiling senescence-associated secretory phenotype in epidermal aging: insights from reversibly immortalized keratinocytes; Authors - Lu-Wen Xu