Alpha 2-Antiplasmin Polyclonal antibody

• 

  WB analysis of HepG2 using 13228-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13228-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13228-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13228-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13228-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13228-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13228-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13228-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 13228-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha 2-Antiplasmin antibody (13228-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Alpha 2-Antiplasmin Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 13228-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Alpha-2-antiplasmin, Serpin F2, ALPHA 2 PI, Alpha 2 AP, Alpha 2 antiplasmin

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Tested Applications

• Positive WB detected in: HepG2 cells, human liver tissue, K-562 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

13228-1-AP targets Alpha 2-Antiplasmin in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4095

  Product name: Recombinant human SERPINF2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 146-491 aa of BC031592

  Sequence: HLLSRLCQDLGPGAFRLAARMYLQKGFPIKEDFLEQSEQLFGAKPVSLTGKQEDDLANINQWVKEATEGKIQEFLSGLPEDTVLLLLNAIHFQGFWRNKFDPSLTQRDSFHLDEQFTVPVEMMQARTYPLRWFLLEQPEIQVAHFPFKNNMSFVVLVPTHFEWNVSQVLANLSWDTLHPPLVWERPTKVRLPKLYLKHQMDLVATLSQLGLQELFQAPDLRGISEQSLVVSGVQHQSTLELSEVGVEAAAATSIAMSRMSLSSFSVNRPFLFFIFEDTTGLPLFVGSVRNPNPSAPRELKEQQDSPGNKDFLQSLKGFPRGDKLFGPDLKLVPPMEEDYPQFGSPK

• Full Name: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
• Calculated Molecular Weight: 491 aa, 55 kDa
• Observed Molecular Weight: 48-55 kDa
• GenBank Accession Number: BC031592
• Gene Symbol: Alpha-2-antiplasmin
• Gene ID (NCBI): 5345
• RRID: AB_2187194
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08697
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Dis Markers; Identification of differentially expressed serum proteins in infectious purpura fulminans.; Authors - Ting He
human	WB	Res Pract Thromb Haemost; Citrullination of α2-antiplasmin is unlikely to contribute to enhanced plasmin generation in COVID-19 pathophysiology; Authors - Kadri Kangro