SETD1A Monoclonal antibody

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SETD1A Monoclonal Antibody for WB, ELISA

Cat No. 67936-1-Ig Copied!

Clone No. 1C2G12

Publications (7)

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Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF, ELISA

Synonyms

hSET1A, KIAA0339, KMT2F, Lysine N methyltransferase 2F, SET domain containing 1A, Set1, Set1A, SETD1A

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  WB analysis using 67936-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 67936-1-Ig

  WB result of SETD1A antibody (67936-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SETD1A transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 67936-1-Ig

  Mouse brain tissue was subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at a dilution of 1:3000 and incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of LNCaP using 67936-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 67936-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HSC-T6 using 67936-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 67936-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, LNCaP cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, mouse brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67936-1-Ig targets SETD1A in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30424

  Product name: Recombinant human SETD1A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-160 aa of NM_014712

  Sequence: MDQEGGGDGQKAPSFQWRNYKLIVDPALDPALRRPSQKVYRYDGVHFSVNDSKYIPVEDLQDPRCHVRSKNRDFSLPVPKFKLDEFYIGQIPLKEVTFARLNDNVRETFLKDMCRKYGEVEEVEILLHPRTRKHLGLARVLFTSTRGAKETVKNLHLTSV

• Full Name: SET domain containing 1A
• Calculated Molecular Weight: 186 kDa
• Observed Molecular Weight: 270 kDa
• GenBank Accession Number: NM_014712
• Gene Symbol: SETD1A
• Gene ID (NCBI): 9739
• RRID: AB_2918688
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O15047
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SETD1A is a SET domain-containing methyltransferase involved in the epigenetic regulation of transcription(PMID:12670868). It is the main catalytic component of a multiprotein complex that methylates lysine 4 of histone H3, a histone mark associated with gene activation (PMID:29937342). Responsible for H3K4me3 enriched promoters and transcriptional programming of inner mass stem cells and neuron progenitors during embryogenesis (PMID:31197650). Does not methylate 'Lys-4' of histone H3 if the neighboring 'Lys-9' residue is already methylated (PubMed:12670868). SETD1A has a calculated molecular weight of 186 kDa, and a 258 kDa band was also reported (PMID: 31076518).

Publications

What published studies show

SETD1A Monoclonal antibody (1C2G12), catalog no. 67936-1-Ig, has 7 total citations appearing in Signal Transduction and Targeted Therapy, Nature Communications, Cell Death & Differentiation, Cancer Letters, Journal of Inflammation, International Journal of Molecular Sciences, and American Journal of Pathology. Research fields include epigenetics, histone H3K4me3 modification, cancer biology (lung adenocarcinoma, colorectal cancer, renal cell carcinoma), autophagy, inflammation, and immunometabolism.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Signal Transduct Target Ther; CXXC finger protein 1 (CFP1) bridges the reshaping of genomic H3K4me3 signature to the advancement of lung adenocarcinoma; Authors - Tao Fan
human,mouse	WB	Nat Commun; Early-life exercise induces immunometabolic epigenetic modification enhancing anti-inflammatory immunity in middle-aged male mice; Authors - Nini Zhang
human	WB	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
human	WB	Cancer Lett; PARP1 interacts with WDR5 to enhance target gene recognition and facilitate tumorigenesis; Authors - Yali Qin
human	IF	J Inflamm (Lond); Histone H3 posttranslational modified enzymes defined neutrophil plasticity and their vulnerability to IL-10 in the course of the inflammation; Authors - Paweł Piatek
human	WB	Int J Mol Sci; H3K4me3 and H3K27ac Promote ccRCC Proliferation Through the CDC6-EXOSC5 Axis.; Authors - Peng Cui ; KD Validated