SETD1A Monoclonal antibody, PBS Only

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  WB analysis using 67936-1-Ig (same clone as 67936-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67936-1-Ig (same clone as 67936-1-PBS)

  WB result of SETD1A antibody (67936-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SETD1A transfected HEK-293 cells. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 67936-1-Ig (same clone as 67936-1-PBS)

  Mouse brain tissue was subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at a dilution of 1:3000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 67936-1-Ig (same clone as 67936-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67936-1-Ig (same clone as 67936-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 67936-1-Ig (same clone as 67936-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67936-1-Ig (same clone as 67936-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SETD1A Monoclonal Antibody for WB, Indirect ELISA

Cat No. 67936-1-PBS

Clone No.1C2G12

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

hSET1A, KIAA0339, KMT2F, Lysine N methyltransferase 2F, SET domain containing 1A, Set1, Set1A, SETD1A

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Product Information

67936-1-PBS targets SETD1A in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30424

  Product name: Recombinant human SETD1A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-160 aa of NM_014712

  Sequence: MDQEGGGDGQKAPSFQWRNYKLIVDPALDPALRRPSQKVYRYDGVHFSVNDSKYIPVEDLQDPRCHVRSKNRDFSLPVPKFKLDEFYIGQIPLKEVTFARLNDNVRETFLKDMCRKYGEVEEVEILLHPRTRKHLGLARVLFTSTRGAKETVKNLHLTSV

• Full Name: SET domain containing 1A
• Calculated Molecular Weight: 186 kDa
• Observed Molecular Weight: 270 kDa
• GenBank Accession Number: NM_014712
• Gene Symbol: SETD1A
• Gene ID (NCBI): 9739
• RRID: AB_2918688
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O15047
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SETD1A is a SET domain-containing methyltransferase involved in the epigenetic regulation of transcription(PMID:12670868). It is the main catalytic component of a multiprotein complex that methylates lysine 4 of histone H3, a histone mark associated with gene activation (PMID:29937342). Responsible for H3K4me3 enriched promoters and transcriptional programming of inner mass stem cells and neuron progenitors during embryogenesis (PMID:31197650). Does not methylate 'Lys-4' of histone H3 if the neighboring 'Lys-9' residue is already methylated (PubMed:12670868). SETD1A has a calculated molecular weight of 186 kDa, and a 258 kDa band was also reported (PMID: 31076518).