SETD2 Polyclonal antibody, PBS Only

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• SETD2 Polyclonal antibody, PBS Only (55377-1-PBS)

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  WB analysis using 55377-1-AP (same clone as 55377-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55377-1-AP (SETD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55377-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS cells using 55377-1-AP (same clone as 55377-1-PBS)

  WB result of SETD2 antibody (55377-1-AP, 1:500) with siRNA transfected U2-OS cells. This data was developed using the same antibody clone with 55377-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 55377-1-AP (same clone as 55377-1-PBS)

  WB result of SETD2 antibody (55377-1-AP; 1:400; room temperature for 1.5 hours) with wild-type and SETD2 knockout Jurkat cells. This data was developed using the same antibody clone with 55377-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 55377-1-AP (same clone as 55377-1-PBS)

  IP result of anti-SETD2 (IP:55377-1-AP, 4ug; Detection:55377-1-AP 1:800) with Jurkat cells lysate 1360 ug. This data was developed using the same antibody clone with 55377-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 55377-1-AP (same clone as 55377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55377-1-AP (SETD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55377-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 55377-1-AP (same clone as 55377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 55377-1-AP (SETD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55377-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 55377-1-AP (same clone as 55377-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SETD2 antibody (55377-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55377-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SETD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 55377-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EC:2.1.1.-, EC:2.1.1.359, HIF 1, HIF1, HIF-1

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Product Information

55377-1-PBS targets SETD2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: SET domain containing 2
• Calculated Molecular Weight: 288 kDa
• Observed Molecular Weight: 287 kDa
• GenBank Accession Number: NM_014159
• Gene Symbol: SETD2
• Gene ID (NCBI): 29072
• RRID: AB_2881318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BYW2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SETD2, also named as HIF1, HYPB, KIAA1732, KMT3A, SET2, p231HBP, hSET2 and HIP-1, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is a histone methyltransferase that methylates 'Lys-36' of histone H3. SETD2 probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. It binds DNA at promoters. SETD2 may also act as a transcription activator that binds to promoters.(PMID:16118227) It is a potential tumor suppressor gene in breast cancer.(PMID:20944102) This antibody is specific to SETD2.