SETD6 Polyclonal antibody

SETD6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24377-1-AP

Synonyms

SET domain containing 6, SETD6

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Validation Data Gallery

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  WB analysis of RAW 264.7 using 24377-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 24377-1-AP (SETD6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24377-1-AP is SETD6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SETD6 fusion protein Ag19485
• Full Name: SET domain containing 6
• Calculated Molecular Weight: 473 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC022451
• Gene Symbol: SETD6
• Gene ID (NCBI): 79918
• RRID: AB_2879514
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SETD6 also named as SET domain containing 6 is a 473 amino-acid protein, which localizes in the Nucleus. The molecular weight of SETD6 is 53kDa. SETD6 consists of 1 SET domain and belongs to the class V-like SAM-binding methyltransferase superfamily. SETD6 as a protein-lysine N-methyltransferase that specifically monomethylates at Lysine 310 of the RELA subunit of NF-kappa-B complex, leading to down-regulate NF-kappa-B transcription factor activity.