SETD7 Polyclonal antibody

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• SETD7 Polyclonal antibody (24840-1-AP)

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SETD7 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 24840-1-AP Copied!

Publications (14)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

EC:2.1.1.364, H3 K4 HMTase SETD7, H3-K4-HMTase SETD7, Histone H3-K4 methyltransferase SETD7, Histone-lysine N-methyltransferase SETD7

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  WB analysis using 24840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 24840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of C6 using 24840-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 24840-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 24840-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 24840-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 24840-1-AP (SETD7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, C6 cells, mouse brain tissue, mouse kidney tissue, L02 cells, rat brain tissue, NIH/3T3 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24840-1-AP targets SETD7 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18219

  Product name: Recombinant human SETD7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-366 aa of BC121055

  Sequence: MDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK

• Full Name: SET domain containing (lysine methyltransferase) 7
• Calculated Molecular Weight: 366 aa, 41 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC121055
• Gene Symbol: SETD7
• Gene ID (NCBI): 80854
• RRID: AB_2737406
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WTS6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SET domain containing lysine methyltransferase 7 (SETD7, also called SET7/9) was the first lysine methyltransferase (KMT) discovered to specifically monomethylate lysine-4 of histone 3 (H3K4me1) (PMID: 26435321). SETD7 has been shown to have a very broad target specificity in vitro, including transcriptional regulators such as TAF10, p53, ER, p65, STAT3, Rb, Mypt, Tat, and Foxo3 (PMID: 25136132, 30013796).

Publications

What published studies show

The SETD7 Polyclonal antibody (Cat# 24840-1-AP) has 14 citations. These appear in journals including Bioactive Materials, Cell Death & Disease, Molecular Therapy, eBioMedicine, Acta Pharmacologica Sinica, Oncotarget, FASEB Journal, Journal of Biological Chemistry, and Biochemical & Biophysical Research Communications. Research fields span epigenetics and histone methylation, cancer (gastric, colorectal, breast, ovarian, pancreatic, osteosarcoma), stem cell differentiation, liver failure, cardiomyocyte hypertrophy, osteoarthritis, and preeclampsia.

Automatically generated from the 14 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Bioact Mater; Dynamic hydrogels orchestrate the differentiation fate of mesenchymal stem cells through epigenetic regulation of SETD7 to accelerate bone defect repair.; Authors - Xudong Xie
human	WB,IHC	Cell Death Dis; ACAT2 suppresses the ubiquitination of YAP1 to enhance the proliferation and metastasis ability of gastric cancer via the upregulation of SETD7; Authors - Mengmeng Zhang ; KD Validated
mouse	WB,IHC,CoIP	Mol Ther; Integrated stress response promotes acute liver failure by activating SETD7 and enhancing NLRP3 methylation.; Authors - Zhentian Nie
human	WB,IHC	EBioMedicine; Histone-lysine N-methyltransferase SETD7 is a potential serum biomarker for colorectal cancer patients.; Authors - Baojun Duan
rat,mouse	WB	Acta Pharmacol Sin; Inhibition of Setd7 protects against cardiomyocyte hypertrophy via inhibiting lipid oxidation.; Authors - Hai-Bi Su ; KD Validated
		Biol Direct; SETD7-mediated epigenetic regulation of LncRNA XIST promotes trophoblast pyroptosis: a study in preeclampsia mouse and cell models.; Authors - Xiaolan Zhao