SEZ6L Recombinant monoclonal antibody

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• SEZ6L Recombinant monoclonal antibody (87923-1-RR)

• 

  WB analysis using 87923-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87923-1-RR (Sez6l antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 87923-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87923-1-RR (SEZ6L antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 87923-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SEZ6L antibody (87923-1-RR, Clone: 260140D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 87923-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SEZ6L antibody (87923-1-RR, Clone: 260140D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SEZ6L Uni-rAb^® Recombinant Antibody for WB, IF-P, ELISA

Cat No. 87923-1-RR

Clone No.260140D4

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

Acupuncture-induced protein 1-L, Aig1l, Brain-specific receptor-like protein B, BSRP-B, Kiaa0927

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue, mouse spinal cord tissue
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87923-1-RR targets SEZ6L in WB, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg9193

  Product name: Recombinant mouse Sez6l protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 32-897 aa of NM_001253916.2

  Sequence: EDGGPEGEMHPSTAYLLPSASLESSLEEGVTSAAPGLLPSQEALEAMEESLPPALPDEASVQHTPALRKGLPSLKQLNSARRQLRPLATPTTLQRLGSPASATTKLREPEDPEQPTAPAPLQIAPFTALATTLPHSPQPAQAPDDSSPGSPLDKGDNELTGSASEESQETTTSTIVTTTIITTEQAPALCGVSFSDPEGYIDSSDFPPQPYSSFLECTYNVTVYTGYGVELQVKSVNLSEGELLSIRGVDGPTLTVLANQTLLVEGQVIRSPTNTISVYFRTFQDDGLGTFQLHYQAFMLSCPFPRRPDAGEVTVMDLHSGGVAHFHCHLGYELQGAKTLTCINASKPHWSSQEPVCSAPCGGAVHNATIGRVLSPSFPGTANGSQLCVWTIEAPEGQKLHLHLERLLLHEKDRMIVYSGRTNTSALLYDSLRTESVPFEGLLSEGSSIRIEFTSDQGQAASAFNIRFEAFEKGHCYEPYIQNGNFTTSDPTYNIGTIVEFTCDPGHSLEQGPAVIECVNVRDPYWNDTEPLCRAMCGGELSAVAGVVLSPNWPEPYAEGEDCVWKIHVGEEKRIFLDIQFLNLSNSDILTIYDGDEVVPHVLGQYFGHSSPQKLYSSTPDLTIQFHSDPAGLIFGKGQGFIMNYIEVSRNDSCSDLPEIQNGWKTTSHTELVRGARITYQCDPGYDIVGSDTLTCQWDLSWSSDPPFCEKIMYCTDPGEVEHSTRLISDPVLLVGTTIQYTCSPGFVLEGSSLLTCYSRETGTPIWTSRLPHCVSEESLACDNPGLPENGYQILYKRLYLPGESLTFMCYEGFELMGEVTIRCILGQPSHWSGPLPICKVNQDSFEHALEAEAAAESSLEGGNMA

• Full Name: seizure related 6 homolog like
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 150-200 kDa
• GenBank Accession Number: NM_001253916.2
• Gene Symbol: Sez6l
• Gene ID (NCBI): 56747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6P1D5-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The BSRPs (brain-specific receptor-like proteins) were predominantly expressed in the soma and dendrites of neurons and localized in the endoplasmic reticulum (ER). Seizure 6-like protein (SEZ6L, also known as BSRP-B) is a member of the BSRP family. It is a candidate tumor suppressor gene and may contribute to specialized endoplasmic reticulum functions in neurons (PMID: 16814779). The observed molecular weight of SEZ6L is higher than its calculated one, because of N-glycosylation (PMID: 27716410).