Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19428-1-AP targets SEZ6L2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13760 Product name: Recombinant human SEZ6L2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 280-626 aa of BC000567 Sequence: RVSLDEDNDRLMVRSGGSPLSPVIYDSDMDDVPERGLISDAQSLYVELLSETPANPLLLSLRFEAFEEDRCFAPFLAHGNVTTTDPEYRPGALATFSCLPGYALEPPGPPNAIECVDPTEPHWNDTEPACKAMCGGELSEPAGVVLSPDWPQSYSPGQDCVWGVHVQEEKRILLQVEILNVREGDMLTLFDGDGPSARVLAQLRGPQPRRRLLSSGPDLTLQFQAPPGPPNPGLGQGFVLHFKEVPRNDTCPELPPPEWGWRTASHGDLIRGTVLTYQCEPGYELLGSDILTCQWDLSWSAAPPACQKIMTCADPGEIANGHRTASDAGFPVGSHVQYRCLPGYSLE Predict reactive species |
| Full Name | seizure related 6 homolog (mouse)-like 2 |
| Calculated Molecular Weight | 910 aa, 98 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC000567 |
| Gene Symbol | SEZ6L2 |
| Gene ID (NCBI) | 26470 |
| RRID | AB_3085577 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UXD5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Seizure 6-like protein 2 (SEZ6L2) is a type 1 transmembrane protein predominantly expressed in the brain. It belongs to the seizure-related gene 6 (SEZ6) family, which contains SEZ6, SEZ6L and SEZ6L2. SEZ6 family members have been shown to influence synapse numbers and dendritic morphology and are linked to various neurological and psychiatric disorders (PMID: 33936031). SEZ6L2 is a part of the AMPA receptor and acts as a scaffolding protein to link GluR1 to adducin (PMID: 29032200). Overexpression of SEZ6L2 has been reported in a variety of cancers, including lung cancer, hepatocellular carcinoma, thyroid carcinoma, cholangiocarcinoma (PMID: 16863507; 35117286; 35284305; 32148567). SEZ6L2 can be glycosylated and the apparent molecular weight can be raised to 150-170 kDa (PMID: 26698217; 24272587).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SEZ6L2 antibody 19428-1-AP | Download protocol |
| WB protocol for SEZ6L2 antibody 19428-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-SEZ6L2 antibody (catalog no. 19428-1-AP) has 1 total citation. It was published in Recent Patents on Anticancer Drug Discovery (IF: 2.4). The study used WB and IHC in human samples to investigate how SEZ6L2 promotes cervical carcinogenesis by activating the Wnt signaling pathway. The associated research field is oncology, specifically cervical cancer and Wnt signaling pathway biology.
| Species | Application | Title |
|---|---|---|
Recent Pat Anticancer Drug Discov SEZ6L2 Promotes Cervical Carcinogenesis by Activating the Wnt Signaling Pathway. |
