SF3A1 Recombinant antibody

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  WB analysis using 86170-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86170-2-RR (SF3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 86170-2-RR

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 86170-2-RR (SF3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86170-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SF3A1 antibody (86170-2-RR, Clone: 250768F4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SF3A1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86170-2-RR

Clone No.250768F4

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

PRP21, SAP 114, SAP114, SF3a120, Spliceosome-associated protein 114

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Tested Applications

• Positive WB detected in: HeLa cells, rat kidney tissue, HEK-293 cells, K-562 cells, HL-60 cells, LNCaP cells, Neuro-2a cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86170-2-RR targets SF3A1 in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SF3A1 fusion protein Ag8196
• Full Name: splicing factor 3a, subunit 1, 120kDa
• Calculated Molecular Weight: 793 aa, 89 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC007684
• Gene Symbol: SF3A1
• Gene ID (NCBI): 10291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15459
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SF3A1 is 88 kDa, but the modified SF3A1 is about 115-120 kDa.(PMID: 7489498 )