SF3B3 Monoclonal antibody

SF3B3 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2H7F5

Cat no : 67469-1-Ig

Synonyms

KIAA0017, RSE1, SAP 130, SAP130, SF3b130, SF3B3, Splicing factor 3B subunit 3, STAF130



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

67469-1-Ig targets SF3B3 in WB, IHC,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SF3B3 fusion protein Ag6315
Full Name splicing factor 3b, subunit 3, 130kDa
Calculated molecular weight 136 kDa
Observed molecular weight 130-135 kDa
GenBank accession numberBC003146
Gene symbol SF3B3
Gene ID (NCBI) 23450
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occured in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which conposed of 4 proteins. SF3B3 is one of SF3B, and it's required for 'A' complex assembly formed by the stable binding of U2 snRNP to the brachpoint sequence(BPS) in pre-mRNA.

Protocols

Product Specific Protocols
WB protocol for SF3B3 antibody 67469-1-IgDownload protocol
IHC protocol for SF3B3 antibody 67469-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols