SF3B4 Recombinant antibody

• 

  WB analysis using 85663-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85663-5-RR (SF3B4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

SF3B4 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85663-5-RR

Clone No.243098D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Pre-mRNA-splicing factor SF3b 49 kDa subunit, SAP 49, SAP49, SF3b49, SF3b50

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, Jurkat cells, U2OS cells, COLO 205 cells, NIH/3T3 cells, C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85663-5-RR targets SF3B4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SF3B4 fusion protein Ag0737
• Full Name: splicing factor 3b, subunit 4, 49kDa
• Calculated Molecular Weight: 44 aa, 3 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC004273
• Gene Symbol: SF3B4
• Gene ID (NCBI): 10262
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15427
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SF3B4, also named as Pre-mRNA-splicing factor SF3b 49 kDa subunit, is a 424 amino acid protein, which belongs to the SF3B4 family. SF3B4 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. The calculated molecular weight of SF3B4 is 44 kDa. Based on its predicted molecular mass and the observed migration of the 50kDa cross-linked species on two-dimensional gels.(PMID: 9614130)