SF4 Monoclonal antibody

SF4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G9D8

Cat no : 68319-1-Ig

Synonyms

DKFZp434E2216, F23858, RBP, RNA binding protein RBP, SF4, splicing factor 4

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Validation Data Gallery

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  WB analysis using 68319-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68319-1-Ig (SF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis of NCCIT using 68319-1-Ig

  NCCIT cells were subjected to SDS PAGE followed by western blot with 68319-1-Ig (SF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: U2OS cells, NCCIT cells, A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68319-1-Ig targets SF4 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SF4 fusion protein Ag8014
• Full Name: splicing factor 4
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC002986
• Gene Symbol: SF4
• Gene ID (NCBI): 57794
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.