SFMBT1 Polyclonal antibody

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  WB analysis using 23631-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23631-1-AP (SFMBT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

SFMBT1 Polyclonal Antibody for WB, ELISA

Cat No. 23631-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

RU1, SFMBT, SFMBT 1

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Tested Applications

• Positive WB detected in: IMR-32 cells, mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23631-1-AP targets SFMBT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SFMBT1 fusion protein Ag20417
• Full Name: Scm-like with four mbt domains 1
• Calculated Molecular Weight: 866 aa, 98 kDa
• Observed Molecular Weight: 93-98 kDa
• GenBank Accession Number: BC014614
• Gene Symbol: SFMBT1
• Gene ID (NCBI): 51460
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q9UHJ3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Scm-like with four MBT domains protein 1 (SFMBT1) is also named as Renal ubiquitous protein 1 (RU1). SFMBT1 is a transcriptional repressor that associates with chromatin and interacts with the N-terminal tail of histone H3 (PMID: 17599839). SFMBT1 forms a complex with Lys-specific demethylase 1 (LSD1) and they are believed to act together in combination with RNA polymerase II in the transcriptional regulation of histone genes (PMID: 23592795). SFMBT1 is up-regulated in CRC tumor tissues and cells and may be associated with drug resistance (PMID: 35577773). SFMBT1 regulates the expression and interacts with key genes involved in heart muscle and artery development (PWWP2A, as well as myogenic differentiation (MYOD, SIX2) (PMID: 35483874). Testes, endocrine glands, and blood are tissues that highly express SFMBT1 (PMID: 25613900). SFMBT1 is also highly expressed in skeletal muscles specifically in undifferentiated myoblasts, and this expression starts diminishing during muscle differentiation (PMID: 23349461).