SFRP2 Polyclonal antibody

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• SFRP2 Polyclonal antibody (12189-1-AP)

SFRP2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 12189-1-AP Copied!

Publications (13)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

FKSG12, SARP1, SDF-5, Secreted apoptosis-related protein 1, Secreted frizzled-related protein 2

• 

  WB analysis using 12189-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12189-1-AP (SFRP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 12189-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12189-1-AP (SFRP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 12189-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SFRP2 antibody (12189-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: rat heart tissue, mouse brain tissue, mouse heart tissue
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12189-1-AP targets SFRP2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2853

  Product name: Recombinant human SFRP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-295 aa of BC008666

  Sequence: MLQGPGSLLLLFLASHCCLGSARGLFLFGQPDFSYKRSNCKPIPVNLQLCHGIEYQNMRLPNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLCVQVKDRCAPVMSAFGFPWPDMLECDRFPQDNDLCIPLASSDHLLPATEEAPKVCEACKNKNDDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERDLKKSVLWLKDSLQCTCEEMNDINAPYLVMGQKQGGELVITSVKRWQKGQREFKRISRSIRKLQC

• Full Name: secreted frizzled-related protein 2
• Calculated Molecular Weight: 295 aa, 34 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC008666
• Gene Symbol: SFRP2
• Gene ID (NCBI): 6423
• RRID: AB_2254520
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96HF1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.

Publications

What published studies show

The anti-SFRP2 antibody (Cat# 12189-1-AP) has 13 total citations spanning journals including Cell Research, British Journal of Cancer, Breast Cancer Research, Scientific Reports, Biomedical Pharmacotherapy, Frontiers in Oncology, Cell Immunology, PLoS One, Biochemical and Biophysical Research Communications, Physiological Genomics, Psychiatry Investigation, Noncoding RNA Research, and Aging Cell. Research fields cover Wnt/β-catenin signaling, hepatocellular carcinoma, thyroid cancer, breast cancer, hair follicle biology, spermatogenesis, hematopoiesis, muscle fibrosis, ulcerative colitis, and autism-related neurobiology.

Automatically generated from the 13 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Res; Comprehensive profiling reveals mechanisms of SOX2-mediated cell fate specification in human ESCs and NPCs.; Authors - Chenlin Zhou
		Br J Cancer; KSRP-mediated Wnt/β-catenin activation promotes follicular thyroid cancer progression and stemness; Authors - Ke-Fan Pan
mouse,human	WB	Breast Cancer Res; Secreted frizzled-related protein 2 monoclonal antibody-mediated IFN-ϒ reprograms tumor-associated macrophages to suppress triple negative breast cancer.; Authors - Lillian Hsu
rabbit	WB	Noncoding RNA Res; Exosomal miRNA-218-5p derived from low-passage dermal papilla cells modulates hair follicle growth and development.; Authors - Bohao Zhao
human	WB	Sci Rep; The role of dexamethasone in mediating the contradictory effects of Wnt antagonists SFRP2 and SFRP3 on human hair follicle growth; Authors - Ons Ben Hamida
human	WB	Biomed Pharmacother; MicroRNA-500a promotes migration and invasion in hepatocellular carcinoma by activating the Wnt/β-catenin signaling pathway.; Authors - Yuntao Guo