Tested Applications
| Positive WB detected in | rat heart tissue, mouse heart tissue |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IF | See 1 publications below |
Product Information
12189-1-AP targets SFRP2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2853 Product name: Recombinant human SFRP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-295 aa of BC008666 Sequence: MLQGPGSLLLLFLASHCCLGSARGLFLFGQPDFSYKRSNCKPIPVNLQLCHGIEYQNMRLPNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLCVQVKDRCAPVMSAFGFPWPDMLECDRFPQDNDLCIPLASSDHLLPATEEAPKVCEACKNKNDDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERDLKKSVLWLKDSLQCTCEEMNDINAPYLVMGQKQGGELVITSVKRWQKGQREFKRISRSIRKLQC Predict reactive species |
| Full Name | secreted frizzled-related protein 2 |
| Calculated Molecular Weight | 295 aa, 34 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC008666 |
| Gene Symbol | SFRP2 |
| Gene ID (NCBI) | 6423 |
| RRID | AB_2254520 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96HF1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SFRP2 antibody 12189-1-AP | Download protocol |
| WB protocol for SFRP2 antibody 12189-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Comprehensive profiling reveals mechanisms of SOX2-mediated cell fate specification in human ESCs and NPCs. | ||
Front Oncol Fucoidan Inhibits the Progression of Hepatocellular Carcinoma via Causing lncRNA LINC00261 Overexpression. | ||
Biomed Pharmacother MicroRNA-500a promotes migration and invasion in hepatocellular carcinoma by activating the Wnt/β-catenin signaling pathway. | ||
PLoS One Digital gene expression tag profiling analysis of the gene expression patterns regulating the early stage of mouse spermatogenesis. | ||
Physiol Genomics Tenotomy Immobilization as a Model to Investigate Skeletal Muscle Fibrosis (with Emphasis on Secreted Frizzled-Related Protein 2). | ||
Psychiatry Investig m6A Methyltransferase METTL3 Reduces Hippocampal Neuron Apoptosis in a Mouse Model of Autism Through the MALAT1/SFRP2/Wnt/β-catenin Axis |
