Tested Applications
| Positive IHC detected in | human cervical cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
This antibody is recommended for IHC.
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10916-1-AP targets SRp20 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1341 Product name: Recombinant human SRp20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-164 aa of BC000914 Sequence: MHRDSCPLDCKVYVGNLGNNGNKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDPRDAADAVRELDGRTLCGCRVRVELSNGEKRSRNRGPPPSWGRRPRDDYRRRSPPPRRRSPRRRSFSRSRSRSLSRDRRRERSLSRERNHKPSRSFSRSRSRSRSNERK Predict reactive species |
| Full Name | splicing factor, arginine/serine-rich 3 |
| Calculated Molecular Weight | 20 kDa |
| GenBank Accession Number | BC000914 |
| Gene Symbol | SRSF3 |
| Gene ID (NCBI) | 6428 |
| RRID | AB_2185064 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P84103 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SFRS3, also named as SRSF3 and SRP20, belongs to the splicing factor SR family. It may be involved in RNA processing in relation with cellular proliferation and/or maturation. SFRS3 is one of the cognate factors. It is a proto-oncogene critical for cell proliferation and tumor induction and maintenance.The MW of SFRS3 is 20kd.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SRp20 antibody 10916-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SRSF3 antibody (Cat# 10916-1-AP) has 8 total citations spanning journals including Gastroenterology, Molecular Cell, EMBO Molecular Medicine, Communications Biology, PLoS Pathogens, Pathology Research and Practice, Frontiers in Oncology, and Revista Portuguesa de Cardiologia. Research fields covered include gastrointestinal stromal tumor subtyping, pluripotency-differentiation regulation, podocyte splicing in glomerulosclerosis, ovarian cancer progression, viral immune evasion, colorectal cancer epigenetics, pan-cancer biomarker analysis, and myocardial fibrosis. Applications include WB and IHC in human and rat samples.
| Species | Application | Title |
|---|---|---|
Gastroenterology Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors | ||
Mol Cell Cross-Regulation between TDP-43 and Paraspeckles Promotes Pluripotency-Differentiation Transition. | ||
EMBO Mol Med Disease-driven post-transcriptional alterations and alternative splicing in podocytes in focal segmental glomerulosclerosis. | ||
Commun Biol IGF2BP3 recruits NUDT21 to regulate SPTBN1 alternative polyadenylation and drive ovarian cancer progression | ||
PLoS Pathog HSV ICP27 hijacks host splicing factor SRSF3 to regulate pre-mRNA splicing and export for viral gene expression and immune evasion.
| ||
Pathol Res Pract LINC01210 promotes malignant phenotypes of colorectal cancer through epigenetically upregulating SRSF3. |
