SGCE Polyclonal antibody

SGCE Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12407-1-AP

Synonyms

ESG, Epsilon-SG, Epsilon-sarcoglycan, Epsilon SG, Epsilon sarcoglycan

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Validation Data Gallery

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  WB analysis of mouse brain using 12407-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12407-1-AP (SGCE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12407-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12407-1-AP (SGCE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12407-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12407-1-AP (SGCE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12407-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12407-1-AP (SGCE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 12407-1-AP

  IP result of anti-SGCE (IP:12407-1-AP, 4ug; Detection:12407-1-AP 1:500) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human stomach cancer using 12407-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12407-1-AP (SGCE antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, human heart tissue, mouse heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12407-1-AP targets SGCE in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SGCE fusion protein Ag3118
• Full Name: sarcoglycan, epsilon
• Calculated Molecular Weight: 437 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC021709
• Gene Symbol: SGCE
• Gene ID (NCBI): 8910
• RRID: AB_2190443
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	J Biochem; Earlier onset of motor deficits in mice with double mutations in Dyt1 and Sgce.; Authors - Fumiaki Yokoi