SGLT2 Polyclonal antibody, PBS Only

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  WB analysis using 24654-1-AP (same clone as 24654-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24654-1-AP (SGLT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 24654-1-AP (same clone as 24654-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 24654-1-AP (SGLT2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 24654-1-AP (same clone as 24654-1-PBS)

  Rat kidney tissue were subjected to SDS PAGE followed by western blot with 24654-1-AP (SGLT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 24654-1-AP (same clone as 24654-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 24654-1-AP (same clone as 24654-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 24654-1-AP (same clone as 24654-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 24654-1-AP (SGLT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 24654-1-AP (same clone as 24654-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 24654-1-AP (same clone as 24654-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 24654-1-AP (same clone as 24654-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 24654-1-AP (same clone as 24654-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 24654-1-AP (SGLT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse kidney using 24654-1-AP (same clone as 24654-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SGLT2 antibody (24654-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24654-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SGLT2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 24654-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

SLC5A2, Na(+)/glucose cotransporter 2, Low affinity sodium-glucose cotransporter

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Product Information

24654-1-PBS targets SGLT2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19163

  Product name: Recombinant human SLC5A2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 180-311 aa of BC131542

  Sequence: ALLGITMIYTVTGGLAALMYTDTVQTFVILGGACILMGYAFHEVGGYSGLFDKYLGAATSLTVSEDPAVGNISSFCYRPRPDSYHLLRHPVTGDLPWPALLLGLTIVSGWYWCSDQVIVQRCLAGKSLTHIK

• Full Name: solute carrier family 5 (sodium/glucose cotransporter), member 2
• Calculated Molecular Weight: 672 aa, 73 kDa
• Observed Molecular Weight: 69-70 kDa
• GenBank Accession Number: BC131542
• Gene Symbol: SGLT2
• Gene ID (NCBI): 6524
• RRID: AB_2750601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P31639
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Sodium-glucose cotransporter 2 (SGLT2), encoded by SLC5A2, utilizes the electrochemical sodium gradient to transport glucose against the cell's internal concentration gradient. Mainly expressed on brush border membrane (BBM) of epithelial cells in the early segment of the proximal tubule, SGLT2 mediates most of the glucose reabsorption by the kidney overall. Inhibition of SGLT2 could improve glucose homeostasis of diabetic patients, which has been considered as a novel strategy for diabetes treatment.