SGLT2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SGLT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

37

Reviews (1)

Cat no : 24654-1-AP

Synonyms

Na(+)/glucose cotransporter 2, SGLT2, SLC5A2, Sodium/glucose cotransporter 2

---

Validation Data Gallery

• 

  WB analysis using 24654-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24654-1-AP (SGLT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 24654-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 24654-1-AP (SGLT2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24654-1-AP

  Rat kidney tissue were subjected to SDS PAGE followed by western blot with 24654-1-AP (SGLT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 24654-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 24654-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat kidney using 24654-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 24654-1-AP (SGLT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 24654-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 24654-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 24654-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat kidney using 24654-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 24654-1-AP (SGLT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse kidney tissue, A549 cells, 37°C incubated mouse kidney, rat kidney tissue
• Positive IHC detected in: human kidney tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IHC: See 14 publications below
• IF: See 11 publications below
• IP: See 1 publications below

Product Information

24654-1-AP targets SGLT2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SGLT2 fusion protein Ag19163
• Full Name: solute carrier family 5 (sodium/glucose cotransporter), member 2
• Calculated Molecular Weight: 672 aa, 73 kDa
• Observed Molecular Weight: 69-70 kDa
• GenBank Accession Number: BC131542
• Gene Symbol: SGLT2
• Gene ID (NCBI): 6524
• RRID: AB_2750601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sodium-glucose cotransporter 2 (SGLT2), encoded by SLC5A2, utilizes the electrochemical sodium gradient to transport glucose against the cell's internal concentration gradient. Mainly expressed on brush border membrane (BBM) of epithelial cells in the early segment of the proximal tubule, SGLT2 mediates most of the glucose reabsorption by the kidney overall. Inhibition of SGLT2 could improve glucose homeostasis of diabetic patients, which has been considered as a novel strategy for diabetes treatment.

Publications

Species	Application	Title
mouse	WB	Circulation; Sodium Glucose Cotransporter-2 Inhibitor Empagliflozin Reduces Infarct Size Independently of Sodium Glucose Cotransporter-2; Authors - Sha Chen
human	IHC	Cancer Lett; SGLT2 promotes pancreatic cancer progression by activating the Hippo signaling pathway via the hnRNPK-YAP1 axis.; Authors - Dianyun Ren
human	WB	Mol Metab; Sodium-glucose co-transporter2 expression and inflammatory activity in diabetic atherosclerotic plaques: Effects of sodium-glucose co-transporter2 inhibitor treatment.; Authors - Nunzia D'Onofrio ; KD Validated
mouse	IHC	Int J Mol Sci; Redefining the Role of ADAM17 in Renal Proximal Tubular Cells and Its Implications in an Obese Mouse Model of Pre-Diabetes.; Authors - Vanesa Palau
mouse	IF	Metabolism; Sodium-glucose cotransporter 2 inhibition attenuates protein overload in renal proximal tubule via suppression of megalin O-GlcNacylation in progressive diabetic nephropathy.; Authors - Hitomi Otomo
human	WB	Front Pharmacol; Empagliflozin Alleviates Hepatic Steatosis by Activating the AMPK-TET2-Autophagy Pathway in vivo and in vitro.; Authors - Ting Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Elina (Verified Customer) (03-04-2019)

The antibody is quite specific, used at a 1:200 dilution in mouse kidney. Comparison to a secondary only staining shows some background. Overall it appears to work well in paraffin embedded tissues, (5um thick).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: mouse kidney

SGLT2 Antibody Immunofluorescence, validation (1:200 dilution) in mouse kidney (Cat no:24654-1-AP)