Tested Applications
| Positive WB detected in | mouse kidney tissue, A549 cells, mouse lung tissue, rat kidney tissue, 37°C incubated mouse kidney |
| Positive IHC detected in | human kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24654-1-AP targets SGLT2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19163 Product name: Recombinant human SLC5A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 180-311 aa of BC131542 Sequence: ALLGITMIYTVTGGLAALMYTDTVQTFVILGGACILMGYAFHEVGGYSGLFDKYLGAATSLTVSEDPAVGNISSFCYRPRPDSYHLLRHPVTGDLPWPALLLGLTIVSGWYWCSDQVIVQRCLAGKSLTHIK Predict reactive species |
| Full Name | solute carrier family 5 (sodium/glucose cotransporter), member 2 |
| Calculated Molecular Weight | 672 aa, 73 kDa |
| Observed Molecular Weight | 45-70 kDa |
| GenBank Accession Number | BC131542 |
| Gene Symbol | SGLT2 |
| Gene ID (NCBI) | 6524 |
| RRID | AB_2750601 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P31639 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Sodium-glucose cotransporter 2 (SGLT2), encoded by SLC5A2, utilizes the electrochemical sodium gradient to transport glucose against the cell's internal concentration gradient. Mainly expressed on brush border membrane (BBM) of epithelial cells in the early segment of the proximal tubule, SGLT2 mediates most of the glucose reabsorption by the kidney overall. Inhibition of SGLT2 could improve glucose homeostasis of diabetic patients, which has been considered as a novel strategy for diabetes treatment. The bands between 45 kDa and 70 kDa represent the glycosylated forms of SGLT2 in different levels.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SGLT2 antibody 24654-1-AP | Download protocol |
| IHC protocol for SGLT2 antibody 24654-1-AP | Download protocol |
| WB protocol for SGLT2 antibody 24654-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SGLT2 Polyclonal antibody (Cat# 24654-1-AP) has 63 citations. Publications appear in journals including Circulation, Cell Metabolism, Nature Communications, Journal of Clinical Investigation, Journal of the American Society of Nephrology, EBioMedicine, Cancer Letters, FASEB Journal, iScience, Frontiers in Pharmacology, and others. Research fields span diabetic nephropathy, cardiovascular disease, cancer (pancreatic, breast, glioblastoma, hepatocellular), renal fibrosis, inflammation, neuroprotection, and metabolic disorders, with applications in WB, IHC, IF, and IP.
| Species | Application | Title |
|---|---|---|
Circulation Sodium Glucose Cotransporter-2 Inhibitor Empagliflozin Reduces Infarct Size Independently of Sodium Glucose Cotransporter-2 | ||
Cell Metab High Glucose Triggers Nucleotide Imbalance through O-GlcNAcylation of Key Enzymes and Induces KRAS Mutation in Pancreatic Cells. | ||
Nat Commun A glucose-enriched lung pre-metastatic niche triggered by matrix stiffness-tuned exosomal miRNAs in hepatocellular carcinoma | ||
Exp Mol Med Deficiency of exocyst complex component Exoc5 exacerbates the progression of kidney fibrosis. | ||
Metabolism Sodium-glucose cotransporter 2 inhibition attenuates protein overload in renal proximal tubule via suppression of megalin O-GlcNacylation in progressive diabetic nephropathy. | ||
J Exp Clin Cancer Res A novel FBW7/NFAT1 axis regulates cancer immunity in sunitinib-resistant renal cancer by inducing PD-L1 expression.
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Reviews
What customers say
Both reviewers used the antibody at a 1:200 dilution for immunohistochemistry on kidney tissue. One reported clear membranous staining in paraffin-embedded sections, while the other noted good specificity in mouse kidney with minor background compared to a secondary-only control. Overall, the antibody performed well in 5 µm paraffin-embedded tissues, earning ratings of 5 and 4 stars respectively.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lisa (Verified Customer) (01-22-2026) | Immunohistochemistry SGLT2 antibody Fixation AFA - paraffine Demasquage citrate PH6 1/200 marquage bien membranaire
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Elina (Verified Customer) (03-04-2019) | The antibody is quite specific, used at a 1:200 dilution in mouse kidney. Comparison to a secondary only staining shows some background. Overall it appears to work well in paraffin embedded tissues, (5um thick).
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