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  • KD/KO Validated

SGMS1 Polyclonal antibody

SGMS1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19050-1-AP

Synonyms

hmob33, MOB, MOB1, Protein Mob, SGMS1, SMS1, sphingomyelin synthase 1, TMEM23, Transmembrane protein 23



Tested Applications

Positive WB detected inHT-29 cells, human heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19050-1-AP targets SGMS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGMS1 fusion protein Ag5246
Full Name sphingomyelin synthase 1
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 25 kDa, 49 kDa
GenBank Accession NumberBC042899
Gene Symbol SGMS1
Gene ID (NCBI) 259230
RRIDAB_2188417
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sphingomyelin synthase (SMS), with two isoforms, SMS1 (SGMS1) and SMS2, is a key enzyme involved in the generation and development of SM. SMS can participate in inflammation, atherosclerosis, proliferation, apoptosis, differentiation and other functions(PMID:30535436). SMS1 is frequently downregulated in various solid cancers, more particularly in melanoma(PMID:31114500). SGMS1 has two isoforms.

Protocols

Product Specific Protocols
WB protocol for SGMS1 antibody 19050-1-APDownload protocol
IHC protocol for SGMS1 antibody 19050-1-APDownload protocol
IP protocol for SGMS1 antibody 19050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Cancer Res

Aneuploid cell survival relies upon sphingolipid homeostasis.

Authors - Yun-Chi Tang
  • KD Validated
humanIHC

J Clin Endocrinol Metab

Dysregulated sphingolipid metabolism in endometriosis.

Authors - Yie Hou Lee
mouseWB,IHC

FASEB J

Sphingomyelin maintains the cutaneous barrier via regulation of the STAT3 pathway.

Authors - Mariko Komuro
human,mouseWB,IF

Exp Neurol

Inhibition of sphingomyelin synthase 1 ameliorates alzheimer-like pathology in APP/PS1 transgenic mice through promoting lysosomal degradation of BACE1.

Authors - Mei-Hong Lu
  • KD Validated
mouseWB

iScience

The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.

Authors - Tirthankar Sinha
mouseIF

Drug Des Devel Ther

Involvement of Huanglian Jiedu Decoction on Microglia with Abnormal Sphingolipid Metabolism in Alzheimer's Disease.

Authors - Yi-Yu Qi