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  • KD/KO Validated

SGMS1 Polyclonal antibody

SGMS1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19050-1-AP

Synonyms

hmob33, MOB, MOB1, Protein Mob, SGMS1, SMS1, sphingomyelin synthase 1, TMEM23, Transmembrane protein 23



Tested Applications

Positive WB detected inhuman heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

19050-1-AP targets SGMS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGMS1 fusion protein Ag5246
Full Name sphingomyelin synthase 1
Calculated molecular weight 49 kDa
Observed molecular weight 25, 50-55 kDa
GenBank accession numberBC042899
Gene symbol SGMS1
Gene ID (NCBI) 259230
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sphingomyelin synthase (SMS), with two isoforms, SMS1 (SGMS1) and SMS2, is a key enzyme involved in the generation and development of SM. SMS can participate in inflammation, atherosclerosis, proliferation, apoptosis, differentiation and other functions(PMID:30535436). SMS1 is frequently downregulated in various solid cancers, more particularly in melanoma(PMID:31114500). SGMS1 has two isoforms.

Protocols

Product Specific Protocols
WB protocol for SGMS1 antibody 19050-1-APDownload protocol
IHC protocol for SGMS1 antibody 19050-1-APDownload protocol
IP protocol for SGMS1 antibody 19050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Cancer Res

Aneuploid cell survival relies upon sphingolipid homeostasis.

Authors - Yun-Chi Tang
  • KD Validated
humanIHC

J Clin Endocrinol Metab

Dysregulated sphingolipid metabolism in endometriosis.

Authors - Yie Hou Lee
mouseWB,IHC

FASEB J

Sphingomyelin maintains the cutaneous barrier via regulation of the STAT3 pathway.

Authors - Mariko Komuro
human,mouseWB,IF

Exp Neurol

Inhibition of sphingomyelin synthase 1 ameliorates alzheimer-like pathology in APP/PS1 transgenic mice through promoting lysosomal degradation of BACE1.

Authors - Mei-Hong Lu
  • KD Validated
mouseWB

iScience

The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.

Authors - Tirthankar Sinha
mouseIF

Drug Des Devel Ther

Involvement of Huanglian Jiedu Decoction on Microglia with Abnormal Sphingolipid Metabolism in Alzheimer's Disease.

Authors - Yi-Yu Qi