Tested Applications
| Positive WB detected in | HT-29 cells, human heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human liver tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19050-1-AP targets SGMS1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5246 Product name: Recombinant human SGMS1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-145 aa of BC042899 Sequence: MKEVVYWSPKKVADWLLENAMPEYCEPLEHFTGQDLINLTQEDFKKPPLCRVSSDNGQRLLDMIETLKMEHHLEAHKNGHANGHLNIGVDIPTPDGSFSIKIKPNGMPNGYRKEMIKIPMPELERSQYPMEWGKTFLAFLYALSC Predict reactive species |
| Full Name | sphingomyelin synthase 1 |
| Calculated Molecular Weight | 49 kDa |
| Observed Molecular Weight | 25 kDa, 49 kDa |
| GenBank Accession Number | BC042899 |
| Gene Symbol | SGMS1 |
| Gene ID (NCBI) | 259230 |
| RRID | AB_2188417 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86VZ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Sphingomyelin synthase (SMS), with two isoforms, SMS1 (SGMS1) and SMS2, is a key enzyme involved in the generation and development of SM. SMS can participate in inflammation, atherosclerosis, proliferation, apoptosis, differentiation and other functions(PMID:30535436). SMS1 is frequently downregulated in various solid cancers, more particularly in melanoma(PMID:31114500). SGMS1 has two isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SGMS1 antibody 19050-1-AP | Download protocol |
| IP protocol for SGMS1 antibody 19050-1-AP | Download protocol |
| WB protocol for SGMS1 antibody 19050-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Antibody to Sphingomyelin Synthase 2 (SMS2), catalog #19050-1-AP, has 16 citations across journals including Nature Communications, Cancer Research, Oncogene, Advanced Science, Cell Chemical Biology, eLife, FASEB Journal, FEBS Journal, Experimental Neurology, Oncogenesis, Journal of Clinical Endocrinology & Metabolism, iScience, Drug Design Development and Therapy, Journal of Virology, Biomedical Chromatography, and Cellular and Molecular Biology. Research fields span lipid metabolism, cancer, neurodegeneration, virology, cardiology, and dermatology.
| Species | Application | Title |
|---|---|---|
Nat Commun Prosaposin maintains lipid homeostasis in dopamine neurons and counteracts experimental parkinsonism in rodents | ||
Adv Sci (Weinh) Nicotine Reprograms Aging-Related Metabolism and Protects Against Motor Decline in Mice | ||
Oncogene TMSB4Y restrains sphingomyelin synthesis via de novo purine synthesis to exert a tumor suppressor function in male esophageal squamous cell carcinoma | ||
Cell Chem Biol Host specific sphingomyelin is critical for replication of diverse RNA viruses
| ||
Drug Des Devel Ther Involvement of Huanglian Jiedu Decoction on Microglia with Abnormal Sphingolipid Metabolism in Alzheimer's Disease. |
