SGOL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SGOL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 16977-1-AP

Synonyms

hSgo1, NY BR 85, SGO, Sgo1, SGOL1, Shugoshin like 1, shugoshin like 1 (S. pombe)

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Validation Data Gallery

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  WB analysis using 16977-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16977-1-AP (SGOL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 16977-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16977-1-AP (SGOL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, PC-3 cells, NIH/3T3 cells, mouse heart tissue, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

16977-1-AP targets SGOL1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SGOL1 fusion protein Ag9700
• Full Name: shugoshin-like 1 (S. pombe)
• Calculated Molecular Weight: 561aa,64 kDa; 292aa,34 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC017867
• Gene Symbol: SGOL1
• Gene ID (NCBI): 151648
• RRID: AB_2188437
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SGOL1 (Shugoshin 1), a centromeric protein, plays an important role in mitosis (PMID: 23102702).

Publications

Species	Application	Title
mouse	WB, IHC	Carcinogenesis; Tumor-promoting/progressing role of additional chromosome instability in hepatic carcinogenesis in Sgo1 (Shugoshin 1) haploinsufficient mice.; Authors - Hiroshi Y Yamada
human	IHC	Front Oncol; Deciphering the Role of Shugoshin-Like Protein 1 in Lung Adenocarcinoma: A Comprehensive Analysis and In Vitro Study.; Authors - Yixiao Yuan
mouse	IHC	Mol Carcinog; Antagonizing pathways leading to differential dynamics in colon carcinogenesis in Shugoshin1 (Sgo1)-haploinsufficient chromosome instability model.; Authors - Chinthalapally V Rao
human	WB,IHC,IF	BMC Med Genomics; Prognosis and biological function of SGOL1 in clear cell renal cell carcinoma: a multiomics analysis; Authors - Zezhong Yang ; KD Validated
human	WB	Front Oncol; Identification and validation of a potential key gene SGOL1 for poor prognosis in hepatocellular carcinoma based on a bioinformatics approach; Authors - Xiaobin Fei
human	WB,IF	J Mol Cell Biol; Sgo1 interacts with CENP-A to guide accurate chromosome segregation in mitosis; Authors - Fengge Wu ; KD Validated