Tested Applications
| Positive WB detected in | HeLa cells, PC-3 cells, NIH/3T3 cells, mouse heart tissue, rat heart tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16977-1-AP targets SGOL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9700 Product name: Recombinant human SGOL1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-292 aa of BC017867 Sequence: MAKERCLKKSFQDSLEDIKKRMKEKRNKNLAEIGKRRSFIAAPCQIITNTSTLLKNYQDNNKMLVLALENEKSKVKEAQDIILQLRKECYYLTCQLYALKGKLTSQQTVEPAQNQEICSSGMDPNSDDSSRNLFVKDLPQIPLEETELPGQGESFQIEATPPETQQSPHLSLKDITNVSLYPVVKIRRLSLSPKKNKASPAVALPKRRCTASVNYKEPTLASKLRRGDPFTDLCFLNSPIFKQKKDLRRSKKRALEVSPAKEAIFILYYVREFVSRFPDCRKCKLETHICLR Predict reactive species |
| Full Name | shugoshin-like 1 (S. pombe) |
| Calculated Molecular Weight | 561aa,64 kDa; 292aa,34 kDa |
| Observed Molecular Weight | 64 kDa |
| GenBank Accession Number | BC017867 |
| Gene Symbol | SGOL1 |
| Gene ID (NCBI) | 151648 |
| RRID | AB_2188437 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5FBB7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SGOL1 (Shugoshin 1), a centromeric protein, plays an important role in mitosis (PMID: 23102702).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SGOL1 antibody 16977-1-AP | Download protocol |
| WB protocol for SGOL1 antibody 16977-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-SGOL1 (catalog no. 16977-1-AP) has 9 total citations published in journals including Molecular Cell, Cell Death & Disease, J Mol Cell Biol, Carcinogenesis, Mol Carcinogenesis, Frontiers in Oncology, Gene, and BMC Medical Genomics. Associated research fields span cancer biology (hepatocellular carcinoma, lung adenocarcinoma, renal cell carcinoma, mesothelioma, colon carcinogenesis), chromosome segregation in mitosis, RNA m6A modification, tumor chemotherapy resistance, and liquid-liquid phase separation.
| Species | Application | Title |
|---|---|---|
Mol Cell The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance | ||
Cell Death Dis Genome-wide CRISPR screen identifies BUB1 kinase as a druggable vulnerability in malignant pleural mesothelioma | ||
J Mol Cell Biol Sgo1 interacts with CENP-A to guide accurate chromosome segregation in mitosis
| ||
Carcinogenesis Tumor-promoting/progressing role of additional chromosome instability in hepatic carcinogenesis in Sgo1 (Shugoshin 1) haploinsufficient mice. | ||
Mol Carcinog Antagonizing pathways leading to differential dynamics in colon carcinogenesis in Shugoshin1 (Sgo1)-haploinsufficient chromosome instability model. | ||
Front Oncol Identification and validation of a potential key gene SGOL1 for poor prognosis in hepatocellular carcinoma based on a bioinformatics approach |
