Tested Applications
| Positive WB detected in | K-562 cells, HeLa cells, human skeletal muscle tissue, mouse liver tissue, SH-SY5Y cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue, human liver cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11019-2-AP targets SGTA in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1475 Product name: Recombinant human SGTA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC008885 Sequence: MDNKKRLAYAIIQFLHDQLRHGGLSSDAQESLEVAIQCLETAFGVTVEDSDLALPQTLPEIFEAAATGKEMPQDLRSPARTPPSEEDSAEAERLKTEGNEQMKVENFEAAVHFYGKAIELNPANAVYFCNRAAAYSKLGNYAGAVQDCERAICIDPAYSKAYGRMGLALSSLNKHVEAVAYYKKALELDPDNETYKSNLKIAELKLREAPSPTGGVGSFDIAGLLNNPGFMSMASNLMNNPQIQQLMSGMISGGNNPLGTPGTSPSQNDLASLIQAGQQFAQQMQQQNPELIEQLRSQIRSRTPSASNDDQQE Predict reactive species |
| Full Name | small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 34-37 kDa |
| GenBank Accession Number | BC008885 |
| Gene Symbol | SGTA |
| Gene ID (NCBI) | 6449 |
| RRID | AB_2188830 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43765 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a steroid receptor molecular co-chaperone critical in a range of biological processes, including viral infection, cell division, mitosis, and cell cycle checkpoint activation. Overexpression of SGTA has been found in various cancers, including prostate/ovary/testis/liver/lung cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SGTA antibody 11019-2-AP | Download protocol |
| IHC protocol for SGTA antibody 11019-2-AP | Download protocol |
| IP protocol for SGTA antibody 11019-2-AP | Download protocol |
| WB protocol for SGTA antibody 11019-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
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| Species | Application | Title |
|---|---|---|
Neuron A spinal muscular atrophy modifier implicates the SMN protein in SNARE complex assembly at neuromuscular synapses | ||
J Pharm Anal 1,8-cineole ameliorates colon injury by downregulating macrophage M1 polarization via inhibiting the HSP90-NLRP3-SGT1 complex
| ||
Fertil Steril Small glutamine-rich tetratricopeptide repeat-containing protein alpha is present in human ovaries but may not be differentially expressed in relation to polycystic ovary syndrome. | ||
J Cell Biol Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes. | ||
Reviews
What customers say
All three reviews rate the antibody 4–5 stars for Western Blot. Users consistently report a strong, specific band at the predicted ~35–36 kDa size in mouse heart tissue. One reviewer noted some non-specific bands but confirmed the specific band was the strongest. The antibody was used at a 1:1000 dilution. Overall, reviewers found it reliable for detecting the target protein with clear specificity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
WEI (Verified Customer) (03-08-2022) | Specific band at predicted size.
|
WEI (Verified Customer) (11-12-2020) | A strong band at the predicted ~35kDa size.
|
Tom (Verified Customer) (10-09-2020) | I saw a band at ~36kDa using this antibody. There are some non-specific bands present but the specific SGTA band was the strongest band.
|
