SGTA Monoclonal antibody, PBS Only

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  WB analysis using 60305-1-Ig (same clone as 60305-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 60305-1-Ig (same clone as 60305-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 60305-1-Ig (same clone as 60305-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 60305-1-Ig (same clone as 60305-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60305-1-Ig (same clone as 60305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60305-1-Ig (SGTA Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60305-1-Ig (same clone as 60305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60305-1-Ig (SGTA Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60305-1-Ig (same clone as 60305-1-PBS)

  Immunofluorescent analysis of HeLa cells using 60305-1-Ig (SGTA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60305-1-PBS in a different storage buffer formulation.

SGTA Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 60305-1-PBS

Clone No.3B10B11

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Alpha SGT, alphaSGT, hSGT, SGT, SGT1, SGTA, UBP, Vpu binding protein

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Product Information

60305-1-PBS targets SGTA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1475

  Product name: Recombinant human SGTA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-313 aa of BC008885

  Sequence: MDNKKRLAYAIIQFLHDQLRHGGLSSDAQESLEVAIQCLETAFGVTVEDSDLALPQTLPEIFEAAATGKEMPQDLRSPARTPPSEEDSAEAERLKTEGNEQMKVENFEAAVHFYGKAIELNPANAVYFCNRAAAYSKLGNYAGAVQDCERAICIDPAYSKAYGRMGLALSSLNKHVEAVAYYKKALELDPDNETYKSNLKIAELKLREAPSPTGGVGSFDIAGLLNNPGFMSMASNLMNNPQIQQLMSGMISGGNNPLGTPGTSPSQNDLASLIQAGQQFAQQMQQQNPELIEQLRSQIRSRTPSASNDDQQE

• Full Name: small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34-37 kDa
• GenBank Accession Number: BC008885
• Gene Symbol: SGTA
• Gene ID (NCBI): 6449
• RRID: AB_2881419
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43765
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a steroid receptor molecular co-chaperone critical in a range of biological processes, including viral infection, cell division, mitosis, and cell cycle checkpoint activation. Overexpression of SGTA has been found in various cancers, including prostate/ovary/testis/liver/lung cancer.