SH2D3A Polyclonal antibody, PBS Only

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• SH2D3A Polyclonal antibody, PBS Only (15641-1-PBS)

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  WB analysis of HeLa using 15641-1-AP (same clone as 15641-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15641-1-AP (SH2D3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15641-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 15641-1-AP (same clone as 15641-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15641-1-AP (SH2D3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15641-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 15641-1-AP (same clone as 15641-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15641-1-AP (SH2D3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15641-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 15641-1-AP (same clone as 15641-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15641-1-AP (SH2D3A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15641-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15641-1-AP (same clone as 15641-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SH2D3A antibody (15641-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 15641-1-PBS in a different storage buffer formulation.

SH2D3A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 15641-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

UNQ175/PRO201, SH2 domain-containing protein 3A, NSP1, Novel SH2-containing protein 1

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Product Information

15641-1-PBS targets SH2D3A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8132

  Product name: Recombinant human NSP1;SH2D3A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC006281

  Sequence: MQVPQDGEDLAGQPWYHGLLSRQKAEALLQQNGDFLVRASGSRGGNPVISCRWRGSALHFEVFRVALRPRPGRPTALFQLEDEQFPSIPALVHSYMTGRRPLSQATGAVVSRPVTWQGPLRRSFSEDTLMDGPARIEPLRARKWSNSQPADLAHMGRSREDPAGMEASTMPISALPRTSSDPVLLKAPAPLGTVADSLRASDGQLQAKAPTKPPRTPSFELPDASERPPTYCELVPRVPSVQGTSPSQSCPEPEAPWWEAEEDEEEENRCFTRPQAEISFCPHDAPSCLLGPQNRPLEPQVLHTLRGLFLEHHPGSTALHLLLVDCQATGLLGVTRDQRGNMGVSSGLEL

• Full Name: SH2 domain containing 3A
• Calculated Molecular Weight: 576 aa, 63 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC006281
• Gene Symbol: SH2D3A
• Gene ID (NCBI): 10045
• RRID: AB_2286019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BRG2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SH2 domain containing 3A (SH2D3A) Is is a novel SH2-containing protein 1 (NSP1) with proline-rich domains and therefore probably involved in the signal-transduction of some ligand-activated tyrosine kinase receptors. Three phosphorylation sites of NSP1 have been found to date. NSP1 may be located at cell membrane play a role in JNK activation.