SH2D3C Polyclonal antibody

SH2D3C Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12882-1-AP

Synonyms

CHAT, Novel SH2 containing protein 3, NSP3, PRO34088, SH2 domain containing 3C, SH2D3C

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Validation Data Gallery

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  WB analysis of HEK-293 using 12882-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12882-1-AP (SH2D3C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12882-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12882-1-AP (SH2D3C antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 12882-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12882-1-AP (SH2D3C antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IP experiment using 12882-1-AP

  IP result of SH2D3C antibody (12882-1-AP for IP and Detection) with mouse brain tissue lysate.

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  IP experiment of mouse brain using 12882-1-AP

  IP result of anti-SH2D3C (IP:12882-1-AP, 3ug; Detection:12882-1-AP 1:500) with mouse brain tissue lysate 6000ug.

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  IHC staining of human breast cancer using 12882-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12882-1-AP (SH2D3C antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human brain tissue, human placenta tissue
• Positive IP detected in: mouse brain tissue,
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IP: See 1 publications below

Product Information

12882-1-AP targets SH2D3C in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SH2D3C fusion protein Ag3887
• Full Name: SH2 domain containing 3C
• Calculated Molecular Weight: 94 kDa, 86 kDa, 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC027962
• Gene Symbol: SH2D3C
• Gene ID (NCBI): 10044
• RRID: AB_2188993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SH2 domain-containing protein 3C (SH2D3C, or NSP3) gene encodes an adaptor protein and member of a cytoplasmic protein family involved in cell migration. The encoded protein was identified as an Eph receptor-binding protein which may be a positive regulator of TCR signaling (PMID: 10542222). SH2D3C is an adaptor protein that has been implicated in T cell activation and shown to associate with different components of the integrin signaling pathway ex vivo, moreover, its primary in vivo role of Sh2d3c in regulating B cell development and function has also been demonstrated (PMID: 20505138). SH2D3C has 6 isoforms with MW 94, 86, 77 and 55 kDa (refer to UniProt).

Publications

Species	Application	Title
rat	WB,IP	Neuroscience; Up-regulation of NSP3 by oligomeric Aβ accelerates neuronal death through Cas-independent Rap1A activation.; Authors - Fujiya Gomi