SH2D5 Polyclonal antibody, PBS Only

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• SH2D5 Polyclonal antibody, PBS Only (55320-1-PBS)

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  WB analysis of HEK-293 using 55320-1-AP (same clone as 55320-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55320-1-AP (SH2D5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 55320-1-AP (same clone as 55320-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55320-1-AP (SH2D5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 55320-1-AP (same clone as 55320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 55320-1-AP (same clone as 55320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 55320-1-AP (same clone as 55320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 55320-1-AP (same clone as 55320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 55320-1-AP (same clone as 55320-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SH2D5 antibody (55320-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

SH2D5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 55320-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SH2 domain-containing protein 5, SH2 domain containing 5

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Product Information

55320-1-PBS targets SH2D5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: SH2 domain containing 5
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 43-47 kDa
• GenBank Accession Number: NM_001103160
• Gene Symbol: SH2D5
• Gene ID (NCBI): 400745
• RRID: AB_2881307
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6ZV89
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SH2D5, or Src homology 2 domain-containing protein 5, is a mammalian-specific adaptor-like protein that contains an N-terminal phosphotyrosine-binding domain and a C-terminal Src homology 2 (SH2) domain. SH2D5 has been found to interact with the breakpoint cluster region protein, a RacGAP that is also highly expressed in the brain. This interaction occurs between the phosphotyrosine-binding domain of SH2D5 and an NxxF motif located within the N-terminal region of the breakpoint cluster region. Furthermore, SH2D5 has been implicated in the regulation of cell growth in hepatocellular carcinoma cells, where it binds to transketolase (TKT) and promotes the activity of STAT3, a key transcription factor involved in cell proliferation and survival.