SH3GLB2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SH3GLB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15897-1-AP

Synonyms

PP9455, PP6569, PP578, KIAA1848, Endophilin-B2

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Validation Data Gallery

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  WB analysis of HeLa using 15897-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 15897-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15897-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 15897-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15897-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15897-1-AP

  IP result of anti-SH3GLB2 (IP:15897-1-AP, 4ug; Detection:15897-1-AP 1:1000) with HeLa cells lysate 2600ug.

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  IHC staining of human placenta using 15897-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15897-1-AP (SH3GLB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15897-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15897-1-AP (SH3GLB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15897-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15897-1-AP (SH3GLB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15897-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15897-1-AP (SH3GLB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human testis tissue, mouse lung tissue, mouse testis tissue, rat lung tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse brain tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

15897-1-AP targets SH3GLB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SH3GLB2 fusion protein Ag8712
• Full Name: SH3-domain GRB2-like endophilin B2
• Calculated Molecular Weight: 395 aa, 44 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC014635
• Gene Symbol: SH3GLB2
• Gene ID (NCBI): 56904
• RRID: AB_2187269
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SH3GLB2 is an endophilin-related protein family with 395-amino acid protein and contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain. SH3GLB2 is expressed in many tissues, including skeletal muscle, adipocytes, brain, lung, colon, and mammary gland. SH3GLB2 was expressed in the cytoplasm of transfected HeLa cells and was excluded from nuclei(PMID: 11161816).

Publications

Species	Application	Title
human	WB	Brain Pathol; Neuronal Susceptibility to Beta-Amyloid Toxicity and Ischemic Injury Involves Histone Deacetylase-2 Regulation of Endophilin-B1.; Authors - David B Wang
mouse	WB,IF	J Biol Chem; Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection.; Authors - Jacob M Serfass ; KO Validated