SHC3 Polyclonal antibody

SHC3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12436-1-AP

Synonyms

N Shc, Neuronal Shc, NSHC, Protein Rai, RAI, SH2 domain protein C3, SHC transforming protein 3, SHC transforming protein C, SHC3, SHCC

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Validation Data Gallery

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  WB analysis using 12436-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12436-1-AP (SHC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of BxPC-3 using 12436-1-AP

  IP result of anti-SHC3 (IP:12436-1-AP, 4ug; Detection:12436-1-AP 1:300) with BxPC-3 cells lysate 2800ug.

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  IHC staining of mouse pancreas using 12436-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12436-1-AP (SHC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 12436-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12436-1-AP (SHC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: BxPC-3 cells
• Positive IP detected in: BxPC-3 cells
• Positive IHC detected in: mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

12436-1-AP targets SHC3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SHC3 fusion protein Ag3115
• Full Name: SHC (Src homology 2 domain containing) transforming protein 3
• Calculated Molecular Weight: 594 aa, 64 kDa
• Observed Molecular Weight: 50-52 kDa
• GenBank Accession Number: BC026314
• Gene Symbol: SHC3
• Gene ID (NCBI): 53358
• RRID: AB_2301798
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell Proteomics; Lysate microarrays enable high-throughput, quantitative investigations of cellular signaling.; Authors - Mark Sevecka
mouse	IHC	J Hepatocell Carcinoma; The Long Non-Coding RNA AC006329.1 Facilitates Hepatocellular Carcinoma Progression and Metastasis by Regulating miR-127-5p/SHC3/ERK Axis; Authors - Xiang Xu Kong